Probe:	Mm-Bmp7 (407901)
Probe preparation:	Antisense DNA
Visualized with:	Chromogenic
Assay notes:	Semi-quantitative RNAscope analysis.

Structure	Level	Pattern	Image	Note
TS28: dorsal raphe nucleus	Present	Regionally restricted	4A''' , 4A' , 4A'' , 4A	Co-labeling of wild type Bmp7 allele and mutant LacZ allele revealed subpopulations of brain cells that preferentially expression the mutant allele, the wild type allele, and biallelic cells that express both.
TS28: periaqueductal grey	Present	Regionally restricted	4A''' , 4A' , 4A'' , 4A	Co-labeling of wild type Bmp7 allele and mutant LacZ allele revealed subpopulations of brain cells that preferentially expression the mutant allele, the wild type allele, and biallelic cells that express both.

Structure	Level	Pattern	Image	Note
TS28: dorsal raphe nucleus	Present	Regionally restricted	4B , 4B''' , 4B'' , 4B'	Co-labeling of wild type Bmp7 allele and mutant LacZ allele revealed subpopulations of brain cells that preferentially expression the mutant allele, the wild type allele, and biallelic cells that express both.
TS28: periaqueductal grey	Present	Regionally restricted	4B , 4B''' , 4B'' , 4B'	Co-labeling of wild type Bmp7 allele and mutant LacZ allele revealed subpopulations of brain cells that preferentially expression the mutant allele, the wild type allele, and biallelic cells that express both.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5A WT Dominant top , 5A Biallelic top , 5A LacZ Dominant top , 5A WT only top , 5A LacZ Only top	Five general cell classes were observed, including biallelic cells, cells with a mutant or wild type allele bias, and monoallelic cells that only express the mutant or the wild type allele.

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present	Regionally restricted		Positive cells in the dorsal raphe nucleus, arcuate nucleus, and cortex are predominantly monoallelic for either the wild type or mutant allele; a subpopulation of cells exhibit allele expression biases; and a smaller subpopulation exhibit biallelic expression.
TS28: cerebral cortex	Present	Regionally restricted		Positive cells in the dorsal raphe nucleus, arcuate nucleus, and cortex are predominantly monoallelic for either the wild type or mutant allele; a subpopulation of cells exhibit allele expression biases; and a smaller subpopulation exhibit biallelic expression.
TS28: dorsal raphe nucleus	Present	Regionally restricted		Positive cells in the dorsal raphe nucleus, arcuate nucleus, and cortex are predominantly monoallelic for either the wild type or mutant allele; a subpopulation of cells exhibit allele expression biases; and a smaller subpopulation exhibit biallelic expression.
TS28: meninges	Present	Regionally restricted		In contrast, to the cells analyzed in other brain regions, the majority of Bmp7+ meningeal cells are biallelic.

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present	Regionally restricted	5D ARN	Expression is predominantly monoallelic for either the wild type or mutant allele.

Structure	Level	Pattern	Image	Note
TS28: meninges	Present	Regionally restricted	5D Meninges	Most meningeal cells expressed both the wild type Bmp7 and mutant LacZ mRNA in Bmp7+/LacZ mice.

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Strong		S5A
TS22: meninges	Strong		S5A

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Strong		S5B

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Absent		S5B'