En1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:8210459
Gene symbol:	En1
Gene name:	engrailed 1

Antibody:

Anti-En1

Results

Specimen S4G Control: embryonic day 12.5 (more

)
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - En1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord	spinal cord interneuron	Present	Regionally restricted	S4G Control top	Expression was in ventral interneurons (V1).

Specimen S4G PbxMNdelta: embryonic day 12.5; Olig2^tm1(cre)Tmj/Olig2⁺, Pbx1^tm1Koss/Pbx1^tm1Koss, Pbx3^tm1Mlc/Pbx3^tm1Mlc (more

)
Note: Conditional mutant. Double labeled: green - En1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: spinal cord	spinal cord interneuron	Present	Regionally restricted	S4G PbxMNdelta top	The organization and specification of ventral interneurons was not affected in PbxMNdelta mice.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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