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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8206352
Gene symbol: Pbx1
Gene name: pre B cell leukemia homeobox 1
Results
Specimen 1A: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present Regionally restricted 1A Pbx1 Expression was detected in progenitors and postmitotic motor neurons.
Specimen 1B brachial (Foxp1): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 1B Pbx1 Foxp1 Pbx1 colocalizes with Foxp1+ lateral motor column neurons at brachial levels.
Specimen 1B brachial (Lhx3): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted 1B Pbx1 Lhx3 brachial Pbx1 colocalizes with Lhx3+ medial motor column neurons at brachial levels.
Specimen 1B thoracic (Lhx3): embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Lhx3 (assay MGI:8206372).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 1B Pbx1 Lhx3 thoracic Pbx1 colocalizes with Lhx3+ preganglionic motor column and hypaxial motor column neurons at thoracic levels.
Specimen 1D Control: embryonic day 11.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: red - Pbx1; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Present 1D Control
Specimen 1D Pbx1MNdelta: embryonic day 11.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: red - Pbx1; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Absent 1D Pbx1MNdelta Expression was lost in progenitors and postmitotic motor neurons.
Specimen 1D PbxMNdelta: embryonic day 11.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: red - Pbx1; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord spinal cord motor neuron Absent 1D PbxMNdelta Expression was lost in progenitors and postmitotic motor neurons.
Specimen S1A brachial: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region spinal cord motor neuron Present Regionally restricted S1A Pbx1 Isl1/2 brachial Pbx1 is expressed in Isl1/2+ motor neurons.
Specimen S1A thoracic left: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1A Pbx1 Isl1/2 thoracic Pbx1 is expressed in Isl1/2+ motor neurons.
Specimen S1A thoracic right: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S1A Pbx1 Foxp1 thoracic Pbx1 is expressed in Foxp1+ preganglionic motor column neurons.
Specimen S1C rostral brachial: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1C Pbx1 Foxp1 Expression in lateral motor column neurons in the rostral brachial region colocalized with Foxp1.
Specimen S1D caudal brachial: embryonic day 11.5 (more )
Note: Double labeled: red - Pbx1; green - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord brachial region lateral motor column neuron Present Regionally restricted S1D Pbx1 Foxp1 Expression in lateral motor column neurons in the caudal brachial region colocalized with a subset of Foxp1 labeled neurons.
Specimen S2D1 Control rostral: embryonic day 12.5 (more )
Note: Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Present S2D1 rostral Control
Specimen S2D1 Pbx1MNdelta rostral: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Absent S2D1 rostral Pbx1MNdelta
Specimen S2D2 Control: embryonic day 12.5 (more )
Note: Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Present S2D2 Control
Specimen S2D2 Pbx1MNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Absent S2D2 Pbx1MNdelta
Specimen S2D3 Control: embryonic day 12.5 (more )
Note: Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Present S2D3 Control
Specimen S2D3 Pbx1MNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Absent S2D3 Pbx1MNdelta
Specimen S2D4 Control: embryonic day 12.5 (more )
Note: Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Present S2D4 Control
Specimen S2D4 Pbx1MNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Absent S2D4 Pbx1MNdelta
Specimen S2D5 Control caudal: embryonic day 12.5 (more )
Note: Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Present S2D5 caudal Control
Specimen S2D5 Pbx1MNdelta caudal: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss (more )
Note: Conditional mutant. Double labeled: green - Pbx1; red - Foxp1 (assay MGI:8206371).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord lateral motor column neuron Absent S2D5 caudal Pbx1MNdelta
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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