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Gene Expression Data
Assay Details
Assay
Reference: J:181272 Le TT, et al., Requirements for Jag1-Rbpj mediated Notch signaling during early mouse lens development. Dev Dyn. 2012 Mar;241(3):493-504
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8172382
Gene symbol: Foxe3
Gene name: forkhead box E3
Antibody: FOXE3 (C-12): sc-48162
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 3G: embryonic day 14.5; Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Double labeled: red - Foxe3; green - Cdh1 (assay MGI:8172376).
Structure Level Pattern Image Note
TS22: lens anterior epithelium Present 3G Coexpressed with Cdh1.
Specimen 3H: embryonic day 14.5; Rbpjtm1Hon/Rbpj+, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant. Double labeled: red - Foxe3; green - Cdh1 (assay MGI:8172376).
Structure Level Pattern Image Note
TS22: lens anterior epithelium Present 3H Coexpressed with Cdh1, similar as in the control.
Specimen 3I: embryonic day 14.5; Rbpjtm1Hon/Rbpjtm1Hon, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant. Double labeled: red - Foxe3; green - Cdh1 (assay MGI:8172376).
Structure Level Pattern Image Note
TS22: lens anterior epithelium Weak 3I A severely reduced lenses with very few Cdh1+Foxe3+ cells. The percentage of fiber cells (Foxe3neg/total nuclei) was significantly increased in the mutant (quantified in Fig 3M).
Specimen not shown: embryonic day 10.5; Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Quantified in Fig 3M.
Structure Level Pattern Image Note
TS17: lens primordium Present The percentage of fiber cells (Foxe3neg/total nuclei) was about 65%.
Specimen not shown: embryonic day 10.5; Rbpjtm1Hon/Rbpj+, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant. Quantified in Fig 3M.
Structure Level Pattern Image Note
TS17: lens primordium Present The percentage of fiber cells (Foxe3neg/total nuclei) was similar as in the control.
Specimen not shown: embryonic day 10.5; Rbpjtm1Hon/Rbpjtm1Hon, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant. Quantified in Fig 3M.
Structure Level Pattern Image Note
TS17: lens primordium Present The percentage of fiber cells (Foxe3neg/total nuclei) was significantly increased in the mutant.
Specimen not shown: embryonic day 12.5; Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Quantified in Fig 3M.
Structure Level Pattern Image Note
TS20: lens Present The percentage of fiber cells (Foxe3neg/total nuclei) was about 70%.
Specimen not shown: embryonic day 12.5; Rbpjtm1Hon/Rbpj+, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant. Quantified in Fig 3M.
Structure Level Pattern Image Note
TS20: lens Present The percentage of fiber cells (Foxe3neg/total nuclei) was similar as in the control.
Specimen not shown: embryonic day 12.5; Rbpjtm1Hon/Rbpjtm1Hon, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant. Quantified in Fig 3M.
Structure Level Pattern Image Note
TS20: lens Present The percentage of fiber cells (Foxe3neg/total nuclei) was similar as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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