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Gene Expression Data
Assay Details
Assay
Reference: J:245758 Carreno G, et al., Hypothalamic sonic hedgehog is required for cell specification and proliferation of LHX3/LHX4 pituitary embryonic precursors. Development. 2017 Sep 15;144(18):3289-3302
Assay type: RNA in situ
MGI Accession ID: MGI:8169368
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 1A Transcripts were detected in anterior hypothalamus and posterior hypothalamus.
TS15: branchial arch endoderm Present Regionally restricted 1A Transcripts were detected in pharyngeal endoderm.
TS15: future pituitary Absent 1A
Specimen 1B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 1B Transcripts were detected in anterior hypothalamus and posterior hypothalamus.
TS17: branchial arch endoderm Present Regionally restricted 1B Transcripts were detected in pharyngeal endoderm.
TS17: telencephalon Present Regionally restricted 1B Transcripts were detected in ventral telencephalon.
TS17: future pituitary Absent 1B
Specimen 1C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 1C Transcripts were detected in anterior hypothalamus and posterior hypothalamus.
TS19: telencephalon Present Regionally restricted 1C Transcripts were detected in ventral telencephalon.
TS19: future pituitary Absent 1C
Specimen 1D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hypothalamus Present Regionally restricted 1D Transcripts were detected in anterior hypothalamus.
TS20: pituitary gland Absent 1D
Specimen 1E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hypothalamus Present 1E
TS22: pituitary gland Absent 1E
Specimen 1F: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: diencephalon subventricular zone Present Regionally restricted 1F Expression was in the subventricular zone around the 3rd ventricle.
TS26: pituitary gland Absent 1F
Specimen 4A: embryonic day 9.5; Hesx1tm1(cre)Jpmb/Hesx1+, Shhtm2Amc/Shh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 4A Expression is detected in the anterior and posterior hypothalamus in the control embryos.
TS15: Rathke's pouch Absent 4A
Specimen 4B: embryonic day 9.5; Hesx1tm1(cre)Jpmb/Hesx1+, Shhtm2Amc/Shhtm2.1Amc (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 4B Expression was lost in the anterior hypothalamus, but signal was detected in posterior hypothalamus of the mutant embryos.
Specimen 4E: embryonic day 10.5; Hesx1tm1(cre)Jpmb/Hesx1+, Shhtm2Amc/Shh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 4E Expression is detected in the anterior and posterior hypothalamus in the control embryos.
TS17: Rathke's pouch Absent 4E
TS17: branchial arch endoderm Present 4E
Specimen 4F: embryonic day 10.5; Hesx1tm1(cre)Jpmb/Hesx1+, Shhtm2Amc/Shhtm2.1Amc (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 4F Expression was lost in the anterior hypothalamus, but signal was detected in posterior hypothalamus of the mutant embryos.
TS17: Rathke's pouch Present Regionally restricted 4F There is ectopic signal in the posterior region of the developing Rathke's pouch adjacent to the pharyngeal endoderm in the mutant.
Specimen 4M: embryonic day 11.5; Hesx1tm1(cre)Jpmb/Hesx1+, Shhtm2Amc/Shh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 4M Expression is detected in the anterior hypothalamus in the control embryos.
TS19: branchial arch endoderm Present 4M
TS19: oral region ectoderm Present 4M
Specimen 4N: embryonic day 11.5; Hesx1tm1(cre)Jpmb/Hesx1+, Shhtm2Amc/Shhtm2.1Amc (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 4N Expression was lost in the anterior hypothalamus, but signal was detected in posterior hypothalamus of the mutant embryos.
TS19: branchial arch endoderm Present 4N
TS19: oral region ectoderm Present 4N
Specimen 10C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 10C Expression is detected in the hypothalamus in the control embryos.
Specimen S1B: embryonic day 9.0 (more )
Note: 16 somites; midline sagittal section
Structure Level Pattern Image Note
TS14: future diencephalon Present Regionally restricted S1B Transcripts are detected in the midline anterior hypothalamus and posterior hypothalamus.
TS14: branchial arch endoderm Present Regionally restricted S1B Transcripts are detected in the pharyngeal endoderm (PE) abutting the developing Rathke's pouch.
TS14: oral region ectoderm Absent S1B
Specimen S1B': embryonic day 9.0 (more )
Note: 16 somites; medio-lateral section
Structure Level Pattern Image Note
TS14: branchial arch endoderm Present S1B'
TS14: future diencephalon Present S1B'
TS14: oral region ectoderm Absent S1B'
Specimen S1B'': embryonic day 9.0 (more )
Note: 16 somites; lateral section
Structure Level Pattern Image Note
TS14: future diencephalon Present S1B''
TS14: oral region ectoderm Absent S1B''
Specimen S1C: embryonic day 9.5 (more )
Note: 22 somites; midline sagittal section
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S1C Transcripts are detected in the midline anterior hypothalamus and posterior hypothalamus.
TS15: branchial arch endoderm Present Regionally restricted S1C Transcripts are detected in the pharyngeal endoderm (PE) abutting the developing Rathke's pouch.
TS15: oral region ectoderm Absent S1C
Specimen S1C': embryonic day 9.5 (more )
Note: 22 somites; medio-lateral section
Structure Level Pattern Image Note
TS15: diencephalon Present S1C'
TS15: oral region ectoderm Absent S1C'
Specimen S1C'': embryonic day 9.5 (more )
Note: 22 somites; lateral section
Structure Level Pattern Image Note
TS15: diencephalon Present S1C''
TS15: oral region ectoderm Absent S1C''
Specimen S1D: embryonic day 9.5 (more )
Note: 28 somites; midline sagittal section
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S1D Transcripts are detected in the midline anterior hypothalamus and posterior hypothalamus.
TS15: branchial arch endoderm Present Regionally restricted S1D Transcripts are detected in the pharyngeal endoderm (PE) abutting the developing Rathke's pouch.
TS15: oral region ectoderm Absent S1D
Specimen S1D': embryonic day 9.5 (more )
Note: 28 somites; medio-lateral section
Structure Level Pattern Image Note
TS15: diencephalon Present S1D'
TS15: oral region ectoderm Absent S1D'
Specimen S1D'': embryonic day 9.5 (more )
Note: 28 somites; lateral section
Structure Level Pattern Image Note
TS15: diencephalon Present S1D''
TS15: oral region ectoderm Absent S1D''
Specimen S1E: embryonic day 10.0 (more )
Note: 33 somites; midline sagittal section
Structure Level Pattern Image Note
TS16: diencephalon Present S1E
Specimen S1E': embryonic day 10.0 (more )
Note: 33 somites; medio-lateral section
Structure Level Pattern Image Note
TS16: diencephalon Present S1E'
Specimen S1E'': embryonic day 10.0 (more )
Note: 33 somites; lateral section
Structure Level Pattern Image Note
TS16: branchial arch endoderm Weak S1E''
TS16: oral region ectoderm Weak S1E''
Specimen S1F: embryonic day 10.5 (more )
Note: 36 somites; midline sagittal section
Structure Level Pattern Image Note
TS17: diencephalon Present S1F
Specimen S1F': embryonic day 10.5 (more )
Note: 36 somites; medio-lateral section
Structure Level Pattern Image Note
TS17: oral region ectoderm Present S1F'
Specimen S1F'': embryonic day 10.5 (more )
Note: 36 somites; lateral section
Structure Level Pattern Image Note
TS17: oral region ectoderm Present S1F''
Specimen S1G: embryonic day 11.0 (more )
Note: 42 somites; midline sagittal section
Structure Level Pattern Image Note
TS18: diencephalon Present S1G
Specimen S1G': embryonic day 11.0 (more )
Note: 42 somites; medio-lateral section
Structure Level Pattern Image Note
TS18: oral region ectoderm Strong S1G'
Specimen S1G'': embryonic day 11.0 (more )
Note: 42 somites; lateral section
Structure Level Pattern Image Note
TS18: oral region ectoderm Strong S1G''
Specimen S1H: embryonic day 11.5 (more )
Note: 46 somites; midline sagittal section
Structure Level Pattern Image Note
TS19: oral region ectoderm Present S1H
Specimen S1H': embryonic day 11.5 (more )
Note: 46 somites; medio-lateral section
Structure Level Pattern Image Note
TS19: oral region ectoderm Strong S1H'
Specimen S1H'': embryonic day 11.5 (more )
Note: 46 somites; lateral section
Structure Level Pattern Image Note
TS19: oral region ectoderm Strong S1H''
Specimen S12A: embryonic day 10.5; Hesx1tm1(cre)Jpmb/Hesx1+, Ptch1tm1Hahn/Ptch1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S12A Expression is detected in developing hypothalamus.
Specimen S12B: embryonic day 10.5; Hesx1tm1(cre)Jpmb/Hesx1+, Ptch1tm1Hahn/Ptch1tm1Hahn (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S12B Expression in mutant developing hypothalamus is comparable to control.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/09/2025
MGI 6.24 		The Jackson Laboratory