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Gene Expression Data
Assay Details
Assay
Reference: J:178173 Mastracci TL, et al., Nkx2.2 and Arx genetically interact to regulate pancreatic endocrine cell development and endocrine hormone expression. Dev Biol. 2011 Nov 1;359(1):1-11
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8161663
Gene symbol: Ghrl
Gene name: ghrelin
Antibody: anti-ghrelin
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 3C: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 3C
Specimen 3D: postnatal day 0; Arxtm1Gldn/?, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male.
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 3D Similar as in the wild-type (quantified in Fig 3A).
Specimen 3E: postnatal day 0; Nkx2-2tm1Jlr/Nkx2-2tm1Jlr (more )
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 3E Dramatically increased number of positive cells, compared with the wild-type (quantified in Fig 3A).
Specimen 3F: postnatal day 0; Arxtm1Gldn/?, Nkx2-2tm1Jlr/Nkx2-2tm1Jlr, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male.
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 3F Increased number of positive cells, compared with the wild-type, but no significant difference with that of the Nkx2-2 null (quantified in Fig 3A). When imaged with identical exposure time, the intensity of ghrelin expression was decreased compared with the Nkx2-2 null.
Specimen 5C: embryonic day 12.5 (more )
Note: Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic epsilon cell Present 5C No ghrelin+/somatostatin+ cells were seen.
Specimen 5D: embryonic day 12.5; Arxtm1Gldn/?, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male. Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic epsilon cell Present 5D Similar as in the wild-type. The ghrelin+/somatostatin+ cells were rare.
Specimen 5E: embryonic day 12.5; Nkx2-2tm1Jlr/Nkx2-2tm1Jlr (more )
Note: Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic epsilon cell Present 5E Increased number of positive cells compared with the wild-type. No ghrelin+/somatostatin+ cells were seen.
Specimen 5F: embryonic day 12.5; Arxtm1Gldn/?, Nkx2-2tm1Jlr/Nkx2-2tm1Jlr, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male. Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS20: pancreas pancreatic epsilon cell Present 5F The population of ghrelin+/somatostatin+ cells was greatly increased.
Specimen 5G: postnatal day 0 (more )
Note: Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 5G No ghrelin+/somatostatin+ cells were seen.
Specimen 5H: postnatal day 0; Arxtm1Gldn/?, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male. Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 5H Similar as in the wild-type. The ghrelin+/somatostatin+ cells were rare.
Specimen 5I: postnatal day 0; Nkx2-2tm1Jlr/Nkx2-2tm1Jlr (more )
Note: Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 5I Increased number of positive cells compared with the wild-type. No ghrelin+/somatostatin+ cells were seen.
Specimen 5J: postnatal day 0; Arxtm1Gldn/?, Nkx2-2tm1Jlr/Nkx2-2tm1Jlr, Tg(Pdx1-cre)6Tuv/0 (more )
Note: Conditional mutant. Arxtm1Gldn/Arxtm1Gldn in female, Arxtm1Gldn/Y in male. Double labeled: red - Ghrl; green - Sst (assay MGI:8161699).
Structure Cell Type Level Pattern Image Note
TS27: pancreas pancreatic epsilon cell Present 5J The population of ghrelin+/somatostatin+ cells was greatly increased.
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