About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:275030 Ferder IC, et al., Meiotic gatekeeper STRA8 suppresses autophagy by repressing Nr1d1 expression during spermatogenesis in mice. PLoS Genet. 2019 May;15(5):e1008084
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8161505
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-gammaH2AX (Ser139) (05-636; JBW301)
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 9B Stra8-/- gH2ax: postnatal day 10; Stra8tm1Dcp/Stra8tm1Dcp (more )
Note: Double labeled: red - H2ax; green - Sycp3 (assay MGI:8161501).
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 9B Stra8-/-;Nr1d1+/+ , 9B Stra8-/-;Nr1d1+/+ gH2AX Foci of expression were absent.
Specimen 9B Stra8-/-;Nr1d1-/- gH2ax: postnatal day 10; Nr1d1tm1Ven/Nr1d1tm1Ven, Stra8tm1Dcp/Stra8tm1Dcp (more )
Note: Double labeled: red - H2ax; green - Sycp3 (assay MGI:8161501).
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 9B Stra8-/-;Nr1d1-/- , 9B Stra8-/-;Nr1d1-/- gH2AX Foci of expression were present.
Specimen S9C Nr1d1+/+ gH2ax: postnatal day 10 (more )
Note: Double labeled: red - H2ax; green - Sycp3 (assay MGI:8161501).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S9C Nr1d1+/+ gH2AX , S9C Nr1d1+/+
Specimen S9C Nr1d1-/- gH2ax: postnatal day 10; Nr1d1tm1Ven/Nr1d1tm1Ven (more )
Note: Double labeled: red - H2ax; green - Sycp3 (assay MGI:8161501).
Structure Level Pattern Image Note
TS28: germ cell of testis Present S9C Nr1d1-/- , S9C Nr1d1-/- gH2AX Authors report no appreciable effect on meiotic initiation.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/15/2026
MGI 6.29 		The Jackson Laboratory