Probe:	Dlx2 cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Strong		1B
TS21: lateral ganglionic eminence	Strong		1B
TS21: medial ganglionic eminence	Strong		1B
TS21: prethalamus	Strong		1B

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Strong		1C
TS21: medial ganglionic eminence	Strong		1C
TS21: telencephalon	Present	Regionally restricted	1C	Expressed in the peduncular region and the septum.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		1D
TS21: main olfactory bulb	Present		1D
TS21: preoptic area	Present		1D
TS21: brain nucleus	Present	Regionally restricted	1D	Expressed in the suprachiasmatic nucleus.
TS21: hypothalamus	Present	Regionally restricted	1D	Expressed in the preoptic-hypothalamic border.
TS21: telencephalon	Present	Regionally restricted	1D	Expressed in the septum.

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Present		1E
TS21: main olfactory bulb	Present		1E
TS21: medial ganglionic eminence	Present		1E
TS21: preoptic area	Present		1E
TS21: telencephalon	Present	Regionally restricted	1E	Expressed in the peduncular region and the septum.
TS21: hypothalamus	Present	Regionally restricted	1E	Expressed in the preoptic-hypothalamic border.
TS21: lateral ganglionic eminence ventricular zone	Present	Regionally restricted	1E	Expressed in most of the ventricular zone, except the most dorsal domain, the ventral pallium.
TS21: ventral pallium	Absent		1E

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Strong		1F
TS21: medial ganglionic eminence	Strong		1F
TS21: telencephalon	Present	Regionally restricted	1F	Expressed in the septum.
TS21: neocortex	Present		1F

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Strong		1G
TS21: telencephalon	Strong	Regionally restricted	1G	Expressed in the peduncular region.
TS21: neocortex	Present		1G
TS21: preoptic area	Present		1G

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Strong		1H
TS21: prethalamus	Strong		1H
TS21: hypothalamus	Strong	Regionally restricted	1H	Expressed in the preoptic-hypothalamic border.
TS21: brain nucleus	Present	Regionally restricted	1H	Expressed in the supraoptic paraventricular region.
TS21: neocortex	Present		1H

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Present		1I
TS21: prethalamus	Present		1I
TS21: brain nucleus	Strong	Regionally restricted	1I	Expressed in the suprachiasmatic nucleus.
TS21: hypothalamus	Present	Regionally restricted	1I	Expressed in the anterior hypothalamus,
TS21: neocortex	Present		1I

Structure	Level	Pattern	Image	Note
TS21: medial ganglionic eminence ventricular zone	Strong		2A	Strong expression in the progenitor ventricular regions, with scattered expression in the most dorsal domain (pMGE1).
TS21: telencephalon	Strong	Regionally restricted	2A	Expressed in the septum.
TS21: lateral ganglionic eminence ventricular zone	Strong	Regionally restricted	2A	Strong expression in the progenitor ventricular regions.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence ventricular zone	Strong	Regionally restricted	3A	Strong expression in progenitor ventricular regions (pLGE1-4).
TS21: medial ganglionic eminence ventricular zone	Strong	Regionally restricted	3A	The ventricular domain of low expression defines the caudal extension of the pMGE1.
TS21: preoptic area	Strong	Regionally restricted	3A	Scattered expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Strong		4I , 4E
TS21: medial ganglionic eminence	Strong		4I , 4E
TS21: prethalamus	Strong		4I , 4E
TS21: hypothalamus	Strong	Regionally restricted	4I , 4E	Expressed in the progenitor domain from the hypothalamic preoptic area.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Strong		5B
TS21: prethalamus	Strong		5B

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Strong		6A
TS21: medial ganglionic eminence	Strong		6A
TS21: telencephalon	Present	Regionally restricted	6A	Expressed in all septal domains except for pSe5, where only occasional positive cells could be observed.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	S1A	Expressed in the ventricular zone of the lateral and medial ganglionic eminences (pLGE1-4, pMGE1-5). The most dorsal part of the MGE (pMGE1) contained very few positive cells.