Antibody:	Anti-YAP (63.7) sc-101199
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: respiratory tract epithelium	Present	Regionally restricted	2B inset , 2B , 2D , 2D inset	Prominent expression in single layer of columnar epithelial progenitors in laryngopharyngeal region. Expression is nuclear and cytoplasmic. Greatest number of cells with nuclear expression between E10.5 and E11.5

Structure	Level	Pattern	Image	Note
TS19: respiratory tract epithelium	Present	Regionally restricted	2F inset , 2F , 2H , 2H inset	Nuclear expression in single cuboidal layer of epithelial progenitors in vocal fold epithelial lamina. Greatest number of cells with nuclear expression between E10.5 and E11.5
TS19: respiratory tract	Weak	Regionally restricted	2F inset , 2H inset , 2H , 2F	Weak expression in mesenchyme of vocal fold.

Structure	Level	Pattern	Image	Note
TS21: larynx	Present	Regionally restricted	2J inset , 2J , 2L , 2L inset	Expression in both nucleus and cytoplasm of cells in epithelial lamina of vocal fold. Expression predominantly in cytoplasm.

Structure	Level	Pattern	Image	Note
TS23: larynx	Present	Regionally restricted	2N , 2P	Nuclear and cytoplasmic expression in vocal fold epithelium. Expression predominantly in cytoplasm.

Structure	Level	Pattern	Image	Note
TS24: larynx	Present	Regionally restricted	2T , 2R	Nuclear and cytoplasmic expression in vocal fold epithelium. Expression predominantly in cytoplasm.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: vocal folds	epithelial cell	Weak	Regionally restricted	2V , 2X	Predominantly cytoplasmic expression in epithelial cells. Only a few cells with nuclear expression. Overall intensity of expression is weaker compared to earlier time points.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: vocal folds	epithelial cell	Weak	Regionally restricted	3B , 3D	Nuclear and cytoplasmic expression in epithelial cells. Nuclear expression localized in apical compartment. Lower intensity of expression compared to embryonic stages.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: vocal folds	epithelial cell	Weak	Regionally restricted	3H , 3F	Cytoplasmic expression in epithelial cells. Nuclear expression in only a few cells in apical cell layers. . Lower intensity of expression compared to embryonic stages.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: vocal folds	epithelial cell	Weak	Regionally restricted	3J , 3L	Expression shifts from cytoplasm to nucleus in some epithelial cells. Nuclear expression primarily in basal cellular compartment; cytoplasmic expression more luminally. Lower intensity of expression compared to embryonic stages.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: vocal folds	epithelial cell	Weak	Regionally restricted	3N , 3P	Expression shifts from cytoplasm to nucleus in some epithelial cells. Basal cells exhibit prominent nuclear localization of expression. Lower intensity of expression compared to embryonic stages.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: vocal folds	epithelial cell	Weak	Regionally restricted	3R , 3T	Expression primarily localized to cytoplasm in basal and apical cells. Nuclear expression in few cells. . Lower intensity of expression compared to embryonic stages.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: vocal folds	epithelial cell	Weak	Regionally restricted	3V , 3X	Expression primarily localized to cytoplasm. Nuclear expression in few cells. . Lower intensity of expression compared to embryonic stages.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: vocal folds	epithelial cell	Present	Regionally restricted	6C inset , 6C	Expression throughout cytoplasm of epithelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: vocal folds	epithelial cell	Present	Regionally restricted	6D inset , 6D	Expression in nucleus and cytoplasm of epithelial cells.