Probe:	mKirre probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong		1A	Expression most prominent in olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		1B
TS28: caudate-putamen	Present		1B
TS28: neocortex	Present		1B
TS28: telencephalon subventricular zone	Absent		1B

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		1C
TS28: dentate gyrus	Present		1C
TS28: hippocampus	Present		1C
TS28: neocortex	Present		1C
TS28: arcuate nucleus	Present		1C
TS28: dorsomedial hypothalamic nucleus	Present		1C
TS28: lateral hypothalamic area	Present		1C
TS28: ventral lateral geniculate nucleus	Present		1C
TS28: central lateral thalamic nucleus	Present		1C
TS28: central medial thalamic nucleus	Present		1C
TS28: lateral posterior thalamic nucleus	Present		1C
TS28: nucleus reuniens	Present		1C

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		1D	Expression most prominent in cochlear nucleus and cerebellum.
TS28: cochlear nucleus	Strong		1D	Expression most prominent in cochlear nucleus and cerebellum.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	2A	Expression first detected in outermost layer of neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2C	Expression in outer layer of neuroepithelium and in differentiating zones

Structure	Cell Type	Level	Pattern	Image	Note
TS19: hindbrain	neuron	Present	Regionally restricted	2D	Expression in neurons, co-expressed with Tubb3.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2E	No co-expression with Nes.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3A	Expression in caudate-putamen and neocortex.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3B	Expression in neocortex and amygdala.
TS19: diencephalon	Present	Regionally restricted	3B	Expression in geniculate thalamus and hypothalamus.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	3C	Expression in caudate-putamen and piriform cortex.
TS21: hypothalamus	Present		3C
TS21: neocortex	Present		3C
TS21: thalamus	Present		3C

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present		3D
TS21: neocortex	Present		3D
TS21: telencephalon	Present	Regionally restricted	3D	Expression in geniculate body and amygdala.

Structure	Level	Pattern	Image	Note
TS25: caudate-putamen	Present		3E
TS25: neocortex	Present		3E
TS25: piriform cortex	Present		3E
TS25: paraventricular thalamic nucleus	Present		3E

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present		3F
TS25: hypothalamus	Present		3F
TS25: neocortex	Present		3F
TS25: thalamus	Present		3F
TS25: brain nucleus	Present	Regionally restricted	3F	Expression in geniculate body.
TS25: dorsomedial hypothalamic nucleus	Present		3F
TS25: lateral posterior thalamic nucleus	Present		3F
TS25: paraventricular thalamic nucleus	Present		3F
TS25: ventral lateral thalamic nucleus	Present		3F
TS25: hypothalamus lateral zone	Present	Regionally restricted	3F	Expression in lateral hypothalamic nucleus.
TS25: midline thalamic group	Present	Regionally restricted	3F	Expression in reuniens thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Present		4A
TS21: cerebral cortex ventricular layer	Absent		4A	No expression in ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Present	Regionally restricted	4B	Expression predominantly in upper layer of cortical plate.
TS23: cerebral cortex intermediate zone	Absent		4B
TS23: cortical subplate	Absent		4B

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present	Widespread	4C	Expression in almost all layers of cortical plate.
TS25: cerebral cortex intermediate zone	Absent		4C
TS25: cortical subplate	Absent		4C

Structure	Level	Pattern	Image	Note
TS27: cortical plate	Present	Widespread	4D	Expression in almost all layers of cortical plate.
TS27: cerebral cortex intermediate zone	Absent		4D
TS27: cortical subplate	Absent		4D

Structure	Level	Pattern	Image	Note
TS27: cortical layer I	Present		4E
TS27: cortical layer II	Present		4E
TS27: cortical layer III	Present		4E
TS27: cortical layer IV	Present		4E
TS27: cortical layer V	Present		4E
TS27: cortical layer VI	Absent		4E

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present		4F
TS28: cortical layer II	Present		4F
TS28: cortical layer III	Present		4F
TS28: cortical layer IV	Present		4F
TS28: cortical layer V	Present		4F
TS28: cortical layer VI	Absent		4F

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Absent		5A

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	5B	Expression in dentate gyrus, limited to superficial layer.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	interneuron	Present		5B inset

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1		Absent		5C	No expression in CA1-3 subfields.
TS28: hippocampus CA3		Absent		5C	No expression in CA1-3 subfields.
TS28: dentate gyrus	dentate gyrus granule cell	Present		5C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	interneuron	Present		5C inset

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Present	Regionally restricted	6A	Expression first detected in glomerular layer at E15.5.

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb mitral cell layer	Present		6B
TS25: main olfactory bulb prospective glomerular layer	Present		6B

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Absent		6C
TS27: main olfactory bulb granule cell layer	Absent		6C
TS27: main olfactory bulb mitral cell layer	Absent		6C
TS27: main olfactory bulb	Present	Scattered	6C	Expression in scattered cells in subventricular zone of olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		6D
TS28: main olfactory bulb mitral cell layer	Strong		6D

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	7A	First expressed in neuroepithelium and differentiating zone of pons at E11.5

Structure	Level	Pattern	Image	Note
TS21: brainstem nucleus	Present	Regionally restricted	7B	Expression clearly detected in cochlear nucleus, vestibular nucleus and trigeminal nucleus.

Structure	Level	Pattern	Image	Note
TS27: cochlear nucleus	Strong		7C
TS27: trigeminal nucleus	Trace		7C	Expression in vestibular nucleus and trigeminal nucleus declined during postnatal development.
TS27: vestibular nucleus	Trace		7C	Expression in vestibular nucleus and trigeminal nucleus declined during postnatal development.

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present		8A

Structure	Level	Pattern	Image	Note
TS21: cerebellum layer	Present	Regionally restricted	8B	Expression in external germinal layer.
TS21: cerebellum	Present	Regionally restricted	8B	Expression in nuclear transitory zone. No expression in cerebellar neuroepithelium and cortical transitory zone.

Structure	Level	Pattern	Image	Note
TS27: cerebellum molecular layer	Present		8C
TS27: deep cerebellar nucleus	Present		8C
TS27: cerebellum Purkinje cell layer	Absent		8C

Structure	Level	Pattern	Image	Note
TS27: cerebellum layer	Present	Regionally restricted	8C inset	Expression in deep portion of external germinal layer. No expression in proliferating zone.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong		8D
TS28: cerebellum molecular layer	Strong		8D
TS28: cerebellum external granule cell layer	Absent		8D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Absent		8D inset