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Gene Expression Data
Assay Details
Assay
Reference: J:175387 Wu X, et al., Differentiation of the ductal epithelium and smooth muscle in the prostate gland are regulated by the Notch/PTEN-dependent mechanism. Dev Biol. 2011 Aug 15;356(2):337-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7852884
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2G: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-1tm3(cre)Mms/Nkx3-1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: mesenchyme of urogenital sinus Present 2G Expressed in the smooth muscle.
Specimen 2J: postnatal day 3; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-1tm3(cre)Mms/Nkx3-1+ (more )
Note: Conditional mutant. Double labeled: green - Acta2; blue - Cre reporter.
Structure Level Pattern Image Note
TS27: peri-prostatic mesenchyme Present 2J Expressed in the periductal smooth muscle.
Specimen 2L: postnatal day 15; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-1tm3(cre)Mms/Nkx3-1+ (more )
Note: Conditional mutant. Double labeled: green - Acta2; blue - Cre reporter.
Structure Level Pattern Image Note
TS28: peri-prostatic mesenchyme Present 2L Expressed in the periductal smooth muscle.
Specimen 2N: postnatal day 28; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-1tm3(cre)Mms/Nkx3-1+ (more )
Note: Conditional mutant. Double labeled: green - Acta2; blue - Cre reporter.
Structure Level Pattern Image Note
TS28: peri-prostatic mesenchyme Present 2N Expressed in the periductal smooth muscle.
Specimen 4A: postnatal day 14; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Pbsn-cre)4Prb/0 (more )
Note: Conditional mutant. Double labeled: green - Acta2; blue - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS28: smooth muscle of ventral prostate smooth muscle cell of prostate Present 4A lower
Specimen 4F/G: postnatal day 14 (more )
Structure Cell Type Level Pattern Image Note
TS28: smooth muscle of ventral prostate smooth muscle cell of prostate Present 4F , 4G
Specimen 5E: postnatal month 3 (more )
Structure Cell Type Level Pattern Image Note
TS28: prostate gland smooth muscle smooth muscle myoblast Present 5E
Specimen 8I: postnatal day 7; Rbpjtm1Hon/Rbpjtm1Hon (more )
Structure Level Pattern Image Note
TS28: prostate gland smooth muscle Present 8I
Specimen 8J: postnatal day 7; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: prostate gland smooth muscle Present 8J Significantly lower content of positive cells compared with the control. Discontinuous smooth muscle enveloped in the distal regions.
Specimen 9A/C/E: postnatal day 10; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant. Double labeled: green - Acta2; red - Pten (assay MGI:7852889).
Structure Cell Type Level Pattern Image Note
TS28: prostate stroma myofibroblast cell Present 9A , 9C , 9E
TS28: prostate stroma smooth muscle cell Present 9A , 9C , 9E
TS28: prostate stroma smooth muscle myoblast Present 9A , 9C , 9E
Specimen 11D: postnatal day 21; Rbpjtm1Hon/Rbpjtm1Hon (more )
Structure Level Pattern Image Note
TS28: prostate gland smooth muscle Present 11D
Specimen 11H: postnatal day 21; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: prostate gland smooth muscle Present 11H Thin and discontinuous smooth muscle enveloped the ducts in the mutant.
Specimen S1D: postnatal day 15; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Nkx3-1tm3(cre)Mms/Nkx3-1+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: peri-prostatic mesenchyme smooth muscle cell of prostate Present S1D Expressed in the periductal smooth muscle.
Specimen not shown: postnatal day 14 (more )
Structure Cell Type Level Pattern Image Note
TS28: smooth muscle of anterior prostate smooth muscle cell of prostate Present
TS28: smooth muscle of dorsal prostate smooth muscle cell of prostate Present
TS28: smooth muscle of lateral prostate smooth muscle cell of prostate Present
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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