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Gene Expression Data
Assay Details
Assay
Reference: J:175387 Wu X, et al., Differentiation of the ductal epithelium and smooth muscle in the prostate gland are regulated by the Notch/PTEN-dependent mechanism. Dev Biol. 2011 Aug 15;356(2):337-49
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7852889
Gene symbol: Pten
Gene name: phosphatase and tensin homolog
Antibody: Anti-Pten (138G6; #9559)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to other - see notes
Results
Specimen 3O: embryonic day 18.5 (more )
Note: Tyramide signal amplification kit was used.
Structure Level Pattern Image Note
TS26: epithelium of urogenital sinus Present 3O
TS26: mesenchyme of urogenital sinus Present Regionally restricted 3O Expressed in the mesenchyme adjacent to the epithelium.
Specimen 8F: postnatal day 14; Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Tyramide signal amplification kit was used.
Structure Level Pattern Image Note
TS28: prostate epithelium Present 8F
Specimen 8G: postnatal day 14; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant. Tyramide signal amplification kit was used.
Structure Level Pattern Image Note
TS28: prostate epithelium Present 8G About 14% more positive cells, compared with the control.
TS28: peri-prostatic mesenchyme Present Regionally restricted 8G About 32% more positive cells in the periductal mesenchyme, compared with the control.
Specimen 9A/C/E: postnatal day 10; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant. Tyramide signal amplification kit was used. Double labeled: red - Pten; green - Acta2 (assay MGI:7852884).
Structure Cell Type Level Pattern Image Note
TS28: prostate stroma smooth muscle cell Absent 9E , 9C , 9A Do not coexpressed with Acta2.
TS28: prostate stroma smooth muscle myoblast Absent 9E , 9C , 9A Do not coexpressed with Acta2.
Specimen 9B/D/F: postnatal day 10; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant. Tyramide signal amplification kit was used. Double labeled: red - Pten; green - Vim (assay MGI:7853896).
Structure Cell Type Level Pattern Image Note
TS28: prostate stroma fibroblast of subepithelial connective tissue of prostatic gland Strong 9B , 9D , 9F Coexpressed with Vim.
TS28: prostate stroma myofibroblast cell Strong 9B , 9D , 9F Coexpressed with Vim.
Specimen not shown: postnatal day 1; Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Tyramide signal amplification kit was used.
Structure Level Pattern Image Note
TS27: peri-prostatic mesenchyme Present
TS27: prostate epithelium Present
Specimen not shown: postnatal day 1; Nkx3-1tm3(cre)Mms/Nkx3-1+, Rbpjtm1Hon/Rbpjtm1Hon (more )
Note: Conditional mutant. Tyramide signal amplification kit was used.
Structure Level Pattern Image Note
TS27: peri-prostatic mesenchyme Present Reduced expression compared with the control.
TS27: prostate epithelium Present Reduced expression compared with the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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