Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:122415
Michalski N, et al., Molecular characterization of the ankle-link complex in cochlear hair cells and its role in the hair bundle functioning. J Neurosci. 2007 Jun 13;27(24):6478-88
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7852160
|
Gene symbol:
|
Adgrv1
|
Gene name:
|
adhesion G protein-coupled receptor V1
|
Antibody:
|
anti-Vlgr1
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
Results
|
Specimen
1A:
embryonic day 17.5
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: organ of Corti |
Very strong |
Regionally restricted |
1A
,
1A'
|
Expression is at the base of the emerging hair bundles and extends as a fine peripheral band to the neural edge short microvilli.
|
Specimen
1B:
postnatal day 1
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Present |
Regionally restricted |
1B'
,
1B
|
Expression is at the basal end of the hair bundles both in outer and inner hair cells.
|
Specimen
1C:
postnatal day 4
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
1C
,
1C'
|
Expression is in a tight band at the base of stereocilia.
|
Specimen
1D:
postnatal day 8
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 8
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
1D
|
Expression is in a tight band at the base of stereocilia.
|
Specimen
1E:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6J
|
Mutant Allele(s):
|
Adgrv1tm1Msat/Adgrv1tm1Msat
|
Age:
|
postnatal day 8
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Specimen
1F:
postnatal day 12
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
1F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 12
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Specimen
1G:
postnatal day 5
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
1G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
1G
|
Expression is in a tight band at the base of stereocilia.
|
Specimen
4A:
postnatal day 6
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
4A
|
Expression is in a tight band at the base of stereocilia.
|
Specimen
4A':
postnatal day 6; Myo7a4626SB/ Myo7a4626SB
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
4A':
(close )
Genetic Background:
|
involves: BALB/cRl
|
Mutant Allele(s):
|
Myo7a4626SB/Myo7a4626SB
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
4A'
|
Expression is in clumps beneath the apical cell surface of hair cells.
|
Specimen
4A'':
postnatal day 6; Whrnwi/ Whrnwi
(more )
Note: Double labeled: green - Adgrv1; red - Actin.
Specimen
4A'':
(close )
Genetic Background:
|
involves: STOCK a Tyrp1b Myo5ad Oca2p Ednrbs
|
Mutant Allele(s):
|
Whrnwi/Whrnwi
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: green - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
4A''
|
Expression is detected at the very base of stereocilia below the ankle-link.
|
Specimen
S1A:
postnatal day 7
(more )
Note: Triple labeled: blue - Adgrv1; green - Ush2a (assay MGI:7852212); red - Actin.
Specimen
S1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: blue - Adgrv1; green - Ush2a (assay MGI:7852212); red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
S1D
,
S1A
|
Expression is in a tight band at the base of stereocilia.
|
Specimen
S1A':
postnatal day 7
(more )
Note: Triple labeled: blue - Adgrv1; green - Vezt (assay MGI:7852215); red - Actin.
Specimen
S1A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: blue - Adgrv1; green - Vezt (assay MGI:7852215); red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
S1A'
,
S1D'
|
Expression is in a tight band at the base of stereocilia.
|
Specimen
S1A'':
postnatal day 7
(more )
Note: Double labeled: blue - Adgrv1; red - Actin.
Specimen
S1A'':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: blue - Adgrv1; red - Actin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Present |
Regionally restricted |
S1D''
,
S1A''
|
Expression is in a tight band at the base of stereocilia.
|
|