Gene Expression Data
Assay Details
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Assay
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Reference:
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J:122415
Michalski N, et al., Molecular characterization of the ankle-link complex in cochlear hair cells and its role in the hair bundle functioning. J Neurosci. 2007 Jun 13;27(24):6478-88
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7852215
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Gene symbol:
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Vezt
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Gene name:
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vezatin, adherens junctions transmembrane protein
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Antibody:
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anti-mA34
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The authors did not specify which antibody was used MGI:5816074 or MGI:5816076 may have been used instead.
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Results
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Specimen
3B:
postnatal day 6
(more  )
Note: Double labeled: green - Vezt; red - Actin.
Specimen
3B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Vezt; red - Actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
3B
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Expression is at the base of stereocilia and along the kinocilium.
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Specimen
3B':
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Adgrv1tm1Msat/Adgrv1tm1Msat
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Vezt; red - Actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
3B'
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Expression is absent at the base of the hair bundle but remains in the kinocilium in both outer and inner hair cells.
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Specimen
4C:
postnatal day 6
(more )
Note: Double labeled: green - Vezt; red - Actin.
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Vezt; red - Actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
4C
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Expression is at the base of stereocilia and along the kinocilium.
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Specimen
4C':
postnatal day 6; Myo7a4626SB/ Myo7a4626SB
(more )
Note: Double labeled: green - Vezt; red - Actin.
Specimen
4C':
(close )
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Genetic Background:
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involves: BALB/cRl
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Mutant Allele(s):
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Myo7a4626SB/Myo7a4626SB
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Vezt; red - Actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
4C'
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Expression is in clumps beneath the apical cell surface of inner hair cells.
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Specimen
4C'':
postnatal day 6; Whrnwi/ Whrnwi
(more )
Note: Double labeled: green - Vezt; red - Actin.
Specimen
4C'':
(close )
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Genetic Background:
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involves: STOCK a Tyrp1b Myo5ad Oca2p Ednrbs
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Mutant Allele(s):
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Whrnwi/Whrnwi
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Vezt; red - Actin.
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Specimen
S1B':
postnatal day 7
(more )
Note: Triple labeled: green - Vezt; blue - Adgrv1 (assay MGI:7852160); red - Actin.
Specimen
S1B':
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Vezt; blue - Adgrv1 (assay MGI:7852160); red - Actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
S1B'
,
S1D'
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Expression is in a tight band at the base of stereocilia.
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Analysis Tools