Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55056
Jimenez MJ, et al., Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. Mol Cell Biol. 1999 Jun;19(6):4431-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7840090
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Gene symbol:
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Mmp13
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Gene name:
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matrix metallopeptidase 13
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Probe:
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Collagenase 3 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
9e:
embryonic day 18.5
(more  )
Note: Sagittal section of the proximal half of the tibia.
Specimen
9e:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Sagittal section of the proximal half of the tibia.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: epiphyseal plate hypertrophic zone |
hypertrophic chondrocyte |
Present |
Regionally restricted |
9f
,
9e
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Expression was detected in hypertrophic chondrocytes in the most distal portion of the epiphyses.
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| TS26: bone marrow |
osteoblast |
Present |
Regionally restricted |
9e
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Expression was detected in osteoblastic cells localized along forming bone trabeculae present in the bone marrow cavity.
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| TS26: tibia |
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Present |
Regionally restricted |
9e
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Expression was detected in cells from the periosteal bud.
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Specimen
9g:
embryonic day 18.5; Runx2tm1Kish/ Runx2tm1Kish
(more )
Note: Sagittal section of the proximal half of the tibia.
Specimen
9g:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Runx2tm1Kish/Runx2tm1Kish
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Sagittal section of the proximal half of the tibia.
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Specimen
not shown:
embryonic day 18.5; Runx2tm1Kish/Runx2 +
(more )
Note: Sagittal section of the proximal half of the tibia.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Runx2tm1Kish/Runx2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Sagittal section of the proximal half of the tibia.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: epiphyseal plate hypertrophic zone |
hypertrophic chondrocyte |
Present |
Regionally restricted |
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Expression was weaker in the heterozygote hypertrophic chondrocytes in the most distal portion of the epiphyses compared with wild type.
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| TS26: bone marrow |
osteoblast |
Present |
Regionally restricted |
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Expression was weaker in heterozygote osteoblastic cells localized along forming bone trabeculae present in the bone marrow cavity.
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| TS26: tibia |
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Present |
Regionally restricted |
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Expression was weaker in the heterozygote compared with wild type.
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Analysis Tools