Gene Expression Data
Assay Details
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Assay
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Reference:
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J:254597
Bhargava S, et al., The epigenetic modifier Fam208a is required to maintain epiblast cell fitness. Sci Rep. 2017 Aug 24;7(1):9322
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7798006
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Results
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Specimen
1D:
embryonic day 7.75
(more  )
Note: Late headfold stage.
Specimen
1D:
(close  )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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1D
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Expression confined to node.
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Specimen
1D':
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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TasorMommeD6/TasorMommeD6
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late streak/early bud stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Strong |
Regionally restricted |
1D'
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Strongly expressed in both anterior and posterior proximal epiblast, gradually reducing towards distal epiblast.
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| TS10: node |
Absent |
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1D'
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Expression undetectable in node.
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Specimen
3A:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3A:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early streak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Regionally restricted |
3A
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Expression concentrated in posterior epiblast.
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Specimen
3A':
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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TasorMommeD6/TasorMommeD6
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Prestreak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Regionally restricted |
3A'
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Anterior expression fails to be completely down-regulated. Expression remains in anterior epiblast.
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Specimen
3B:
embryonic day 6.75
(more )
Note: Midstreak stage.
Specimen
3B:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Midstreak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo |
Present |
Regionally restricted |
3B
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Expression concentrated in posterior epiblast.
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Specimen
3B':
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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TasorMommeD6/TasorMommeD6
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Prestreak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
Regionally restricted |
3B'
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Anterior expression fails to be completely down-regulated. Expression remains in anterior epiblast
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Analysis Tools