Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:305429 Kaiser M, et al., Regulation of otocyst patterning by Tbx2 and Tbx3 is required for inner ear morphogenesis in the mouse. Development. 2021 Apr 15;148(8):dev195651
Assay type:	RNA in situ
MGI Accession ID:	MGI:7789041
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

7A anterior control Fgf8 ©

Specimen 7A anterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	7A anterior control Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.

7A anterior Tbx2/3cDKO Fgf8 ©

Specimen 7A anterior Tbx2/3cDKO: embryonic day 10.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	7A anterior Tbx2/3cDKO Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.
TS17: otocyst	Present	Regionally restricted	7A anterior Tbx2/3cDKO Fgf8	In Tbx2/3cDKO embryos, ectopic expression was detected in the ventral otocyst along the anterior posterior axis.

7A mid-transverse control Fgf8 ©

Specimen 7A mid-transverse control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		7A mid-transverse control Fgf8

7A mid-transverse Tbx2/3cDKO Fgf8 ©

Specimen 7A mid-transverse Tbx2/3cDKO: embryonic day 10.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	7A mid-transverse Tbx2/3cDKO Fgf8	In Tbx2/3cDKO embryos, ectopic expression was detected in the ventral otocyst along the anterior posterior axis.

7A posterior control Fgf8 ©

Specimen 7A posterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		7A posterior control Fgf8

7A posterior Tbx2/3cDKO Fgf8 ©

Specimen 7A posterior Tbx2/3cDKO: embryonic day 10.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	7A posterior Tbx2/3cDKO Fgf8	In Tbx2/3cDKO embryos, ectopic expression was detected in the ventral otocyst along the anterior posterior axis.

S7A anterior control Fgf8 ©

Specimen S7A anterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	S7A anterior control Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.

S7A anterior Tbx2cKO Fgf8 ©

Specimen S7A anterior Tbx2cKO: embryonic day 10.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	S7A anterior Tbx2cKO Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.
TS17: otocyst	Present	Regionally restricted	S7A anterior Tbx2cKO Fgf8	Ectopic expression was detected in the Tbx2cKO otocyst.

S7A mid-transverse control Fgf8 ©

Specimen S7A mid-transverse control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S7A mid-transverse control Fgf8

S7A mid-transverse Tbx2cKO Fgf8 ©

Specimen S7A mid-transverse Tbx2cKO: embryonic day 10.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	S7A mid-transverse Tbx2cKO Fgf8	Ectopic expression was detected in the Tbx2cKO otocyst.

S7A posterior control Fgf8 ©

Specimen S7A posterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S7A posterior control Fgf8

S7A posterior Tbx2cKO Fgf8 ©

Specimen S7A posterior Tbx2cKO: embryonic day 10.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	S7A posterior Tbx2cKO Fgf8	Ectopic expression was detected in the Tbx2cKO otocyst.

S7B anterior control Fgf8 ©

Specimen S7B anterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	S7B anterior control Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.

S7B anterior Tbx3cKO Fgf8 ©

Specimen S7B anterior Tbx3cKO: embryonic day 10.5; Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	S7B anterior Tbx3cKO Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.
TS17: otocyst	Ambiguous		S7B anterior Tbx3cKO Fgf8	Ectopic expression was not detected in the Tbx3cKO otocyst.

S7B mid-transverse control Fgf8 ©

Specimen S7B mid-transverse control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S7B mid-transverse control Fgf8

S7B mid-transverse Tbx3cKO Fgf8 ©

Specimen S7B mid-transverse Tbx3cKO: embryonic day 10.5; Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S7B mid-transverse Tbx3cKO Fgf8	Ectopic expression was not detected in the Tbx3cKO otocyst.

S7B posterior control Fgf8 ©

Specimen S7B posterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S7B posterior control Fgf8

S7B posterior Tbx3cKO Fgf8 ©

Specimen S7B posterior Tbx3cKO: embryonic day 10.5; Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S7B posterior Tbx3cKO Fgf8	Ectopic expression was not detected in the Tbx3cKO otocyst.

S8_anterior control Fgf8 ©

Specimen S8 anterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	S8_anterior control Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.

S8_anterior Tbx1KO Fgf8 ©

Specimen S8 anterior Tbx1KO: embryonic day 10.5; Tbx1^tm1Pa/Tbx1^tm1Pa (more

)

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	S8_anterior Tbx1KO Fgf8	Expression was restricted to the periphery of the statoacoustic ganglion in the anterior region.
TS17: otocyst	Ambiguous		S8_anterior Tbx1KO Fgf8	Ectopic expression was not detected in the Tbx1KO otocyst.

S8_mid-transverse control Fgf8 ©

Specimen S8 mid-transverse control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S8_mid-transverse control Fgf8

Specimen S8 mid-transverse Tbx1KO: embryonic day 10.5; Tbx1^tm1Pa/Tbx1^tm1Pa (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S8_mid-transverse Tbx1KO Fgf8	Ectopic expression was not detected in the Tbx1KO otocyst.

Specimen S8 posterior control: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S8_posterior control Fgf8

Specimen S8 posterior Tbx1KO: embryonic day 10.5; Tbx1^tm1Pa/Tbx1^tm1Pa (more

)

Structure	Level	Pattern	Image	Note
TS17: otocyst	Ambiguous		S8_posterior Tbx1KO Fgf8	Ectopic expression was not detected in the Tbx1KO otocyst.

Specimen S10A anterior control: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		S10A anterior control Fgf8

Specimen S10A anterior Tbx2/3cDKO: embryonic day 9.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		S10A anterior Tbx2/3cDKO Fgf8

Specimen S10A mid-transverse control: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		S10A mid-transverse control Fgf8

Specimen S10A mid-transverse Tbx2/3cDKO: embryonic day 9.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		S10A mid-transverse Tbx2/3cDKO Fgf8

Specimen S10A posterior control: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		S10A posterior control Fgf8

Specimen S10A posterior Tbx2/3cDKO: embryonic day 9.5; Tbx2^tm2.1Vmc/Tbx2^tm2.1Vmc, Tbx3^tm1Pa/Tbx3^tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Absent		S10A posterior Tbx2/3cDKO Fgf8

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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