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Gene Expression Data
Assay Details
Assay
Reference: J:174095 Cai Z, et al., Loss of shp2-mediated mitogen-activated protein kinase signaling in muller glial cells results in retinal degeneration. Mol Cell Biol. 2011 Jul;31(14):2973-83
Assay type: Western blot
MGI Accession ID: MGI:7788885
Gene symbol: Mapk1
Gene name: mitogen-activated protein kinase 1
Antibody: anti-phospho-ERK1/2 (D13.14.4E; #4370)
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; both family members have been annotated.
Results Image: 6A pERK
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
WT P21 TS28: retina Present Not Specified Not Specified
LSL-Kras-cKO P21 TS28: retina Present (a) involves: 129S4/SvJae * C57BL/6 * DBA/2 Krastm4Tyj/Krastm4Tyj, Tg(Six3-cre)69Frty/0Not Specified Conditional mutant.
Shp2-cKO P21 TS28: retina Weak (b) involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2 Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0Not Specified Conditional mutant.
Shp2/LSL-Kras-cKO P21 TS28: retina Present (c) involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6 * DBA/2 Krastm4Tyj/Krastm4Tyj, Ptpn11tm1Gsf/Ptpn11tm1Gsf, Tg(Six3-cre)69Frty/0Not Specified Conditional mutant.
Notes:
(a) Modestly increased phosphorylation compared with the wild-type.
(b) Reduced phosphorylation compared with the wild-type.
(c) Similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/30/2025
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