Gene Expression Data
Assay Details
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Assay
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Reference:
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J:298691
Wanigasuriya I, et al., Smchd1 is a maternal effect gene required for genomic imprinting. Elife. 2020 Nov 13;9:e55529
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7785354
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Gene symbol:
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Smchd1
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Gene name:
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SMC hinge domain containing 1
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Reporter:
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GFP
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Antibody:
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anti-GFP (#A11122)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1d Smchd1GFP/GFP F x Smchd1+/+ M:
embryonic day 2.5; Smchd1tm1.1Mebl/Smchd1 +
(more  )
Specimen
1d Smchd1 F x Smchd1<+/+> M:
(close  )
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Genetic Background:
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B6.129T2(FVB)-Smchd1tm1.1Mebl
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Mutant Allele(s):
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Smchd1tm1.1Mebl/Smchd1+
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
S1-1c Smchd1 F x Smchd1 M positive control:
(close )
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Genetic Background:
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B6.129T2(FVB)-Smchd1tm1.1Mebl
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Mutant Allele(s):
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Smchd1tm1.1Mebl/Smchd1tm1.1Mebl
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Specimen
S1-1c Smchd1+/+ F x Smchd1GFP/GFP M 8-cell:
embryonic day 2.5; Smchd1tm1.1Mebl/Smchd1 +
(more )
Specimen
S1-1c Smchd1<+/+> F x Smchd1 M 8-cell:
(close )
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Genetic Background:
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B6.129T2(FVB)-Smchd1tm1.1Mebl
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Mutant Allele(s):
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Smchd1tm1.1Mebl/Smchd1+
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Age:
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embryonic day 2.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Weak |
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S1-1c 8-cell
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Using immunofluorescence antibody staining (rather than direct visualization) found very low levels of paternal Smchd1 switching on from the 8-cell stage (66% positive%). The expression is much lower than maternal Smchd1 at this time.
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Specimen
S1-1c Smchd1+/+ F x Smchd1GFP/GFP M 12-16-cell:
embryonic day 2.75; Smchd1tm1.1Mebl/Smchd1 +
(more )
Specimen
S1-1c Smchd1<+/+> F x Smchd1 M 12-16-cell:
(close )
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Genetic Background:
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B6.129T2(FVB)-Smchd1tm1.1Mebl
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Mutant Allele(s):
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Smchd1tm1.1Mebl/Smchd1+
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Age:
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embryonic day 2.75
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Weak |
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S1-1c 12-16 cell
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Using immunofluorescence antibody staining (rather than direct visualization) found very low levels of paternal Smchd1 switching on from the 8-cell stage (100% positive). The expression is much lower than maternal Smchd1 at this time.
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Specimen
S1-1c Smchd1+/+ F x Smchd1GFP/GFP M 24-32-cell:
embryonic day 3.0; Smchd1tm1.1Mebl/Smchd1 +
(more )
Specimen
S1-1c Smchd1<+/+> F x Smchd1 M 24-32-cell:
(close )
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Genetic Background:
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B6.129T2(FVB)-Smchd1tm1.1Mebl
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Mutant Allele(s):
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Smchd1tm1.1Mebl/Smchd1+
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Weak |
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S1-1c 24-32 cell
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Using immunofluorescence antibody staining (rather than direct visualization) found very low levels of paternal Smchd1 switching on from the 8-cell stage (100% positive). The expression is much lower than maternal Smchd1 at this time.
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Analysis Tools