Shh Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:173604 Allen BL, et al., Overlapping Roles and Collective Requirement for the Coreceptors GAS1, CDO, and BOC in SHH Pathway Function. Dev Cell. 2011 Jun 14;20(6):775-87
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7781593
Gene symbol:	Shh
Gene name:	sonic hedgehog

Antibody:	5E1
Detection system:	Secondary antibody

Results

Specimen 2A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2A
TS17: notochord	Present		2A

Specimen 2B: embryonic day 10.5; Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2B	Similar as in the wild-type.
TS17: notochord	Present		2B	Similar as in the wild-type.

Specimen 2C: embryonic day 10.5; Cdon^tm1Rsk/Cdon^tm1Rsk (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2C	Reduced expression compared with the wild-type.
TS17: notochord	Present		2C

Specimen 2D: embryonic day 10.5; Boc^tm2Rsk/Boc^tm2Rsk, Cdon^tm1Rsk/Cdon⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		2D	Similar as in the wild-type.
TS17: notochord	Present		2D	Similar as in the wild-type.

Specimen 2E: embryonic day 10.5; Cdon^tm1Rsk/Cdon^tm1Rsk, Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS17: notochord	Present		2E
TS17: neural tube floor plate	Absent		2E

Specimen 2F: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Absent		2F
TS17: notochord	Present		2F

Specimen 2G: embryonic day 10.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Absent		2G
TS17: notochord	Present		2G

Specimen 2H: embryonic day 10.5; Boc^tm2Rsk/Boc^tm2Rsk, Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Absent		2H
TS17: notochord	Present		2H

Specimen 3A: embryonic day 9.5; Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		3A
TS15: notochord	Present		3A

Specimen 3B: embryonic day 9.5; Cdon^tm1Rsk/Cdon^tm1Rsk, Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Absent		3B
TS15: notochord	Present		3B

Specimen 3C: embryonic day 9.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Absent		3C
TS15: notochord	Present		3C

Specimen 3J: embryonic day 9.5; Cdon^tm1Rsk/Cdon^tm1Rsk, Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Absent		3J
TS15: notochord	Present		3J

Specimen 3K: embryonic day 9.5; Boc^tm2Rsk/Boc^tm2Rsk, Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Absent		3K
TS15: notochord	Present		3K

Specimen 3P: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Strong		3P

Specimen 3P inset: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: notochord	Strong		3P inset

Specimen 3Q: embryonic day 12.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Absent		3Q

Specimen 3Q inset: embryonic day 12.5; Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		3Q inset

Specimen 3R: embryonic day 12.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Absent		3R

Specimen 3R inset: embryonic day 12.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		3R inset

Specimen 7A: embryonic day 9.5; Boc^tm2Rsk/Boc^tm2Rsk, Cdon^tm1Rsk/Cdon⁺, Gas1^tm2Fan/Gas1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		7A	Secreted protein can be detected in the lateral neural tube.
TS15: notochord	Present		7A

Specimen 7B: embryonic day 9.5; Boc^tm2Rsk/Boc^tm2Rsk, Cdon^tm1Rsk/Cdon⁺, Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		7B
TS15: neural tube floor plate	Absent		7B	Secreted protein can be detected in the lateral neural tube.

Specimen 7C: embryonic day 9.5; Boc^tm2Rsk/Boc^tm2Rsk, Cdon^tm1Rsk/Cdon^tm1Rsk, Gas1^tm2Fan/Gas1^tm2Fan (more

)

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		7C
TS15: neural tube lateral wall	Absent		7C	Secreted protein can be detected in the lateral neural tube.

Specimen 7D: embryonic day 9.5; Shh^tm1Amc/Shh^tm1Amc (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		7D
TS15: notochord	Absent		7D

Specimen S1A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1A
TS17: notochord	Present		S1A

Specimen S1D: embryonic day 10.5; Boc^tm2Rsk/Boc⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1D	Similar as in the wild-type.
TS17: notochord	Present		S1D	Similar as in the wild-type.

Specimen S1G: embryonic day 10.5; Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1G	Similar as in the wild-type.
TS17: notochord	Present		S1G	Similar as in the wild-type.

Specimen not shown: embryonic day 8.5; Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube floor plate	Present			Similar as in the wild-type.
TS13: notochord	Present			Similar as in the wild-type.

Specimen not shown: embryonic day 9.5; Boc^tm2Rsk/Boc^tm2Rsk (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present			Similar as in the wild-type.
TS15: notochord	Present			Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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