Gene Expression Data
Assay Details
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Assay
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Reference:
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J:356614
Sommer P, et al., Identification of Tgf beta1i4 as a downstream target of Foxc1. Dev Growth Differ. 2006 Jun;48(5):297-308
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7779850
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Gene symbol:
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Tsc22d1
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Gene name:
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TSC22 domain family, member 1
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Probe:
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Tgfb1i4 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 13.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: head mesenchyme |
Present |
Widespread |
3A
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Widely expressed in head mesenchyme.
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| TS22: conjunctiva epithelium |
Strong |
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3A
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Intense expression in conjunctival epithelium.
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| TS22: eye |
Strong |
Regionally restricted |
3A
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Intense expression in region where future Harderian and lacrimal glands will bud.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Foxc1tm1Blh/Foxc1tm1Blh
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: head mesenchyme |
Present |
Widespread |
3B
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Widely expressed in head mesenchyme.
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| TS22: eye |
Weak |
Regionally restricted |
3B
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Expression appears weaker and more diffuse around temporal region of eye compared to wild type.
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Specimen
3C:
embryonic day 13.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
Present |
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3C
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Expression in presumptive corneal epithelium, with expression extending into conjunctival epithelium
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| TS22: cornea epithelium |
Present |
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3C
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Expression in presumptive corneal epithelium, with expression extending into conjunctival epithelium
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| TS22: eye |
Present |
Regionally restricted |
3C
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Expression in optic cup margin (at site of future ciliary body) and around the optic nerve.
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| TS22: lens epithelium |
Present |
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3C
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Expression in periocular mesenchyme and lens epithelium.
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| TS22: perioptic mesenchyme |
Present |
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3C
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Expression in periocular mesenchyme and lens epithelium.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Foxc1tm1Blh/Foxc1tm1Blh
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
Strong |
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3D
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More intense expression in conjunctival epithelium compared to wild type.
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| TS22: face mesenchyme |
Strong |
Regionally restricted |
3D
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Intense expression in condensing mesenchyme on nasal aspect of eye surrounding postoral vibrissa.
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Specimen
3E:
embryonic day 13.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
Present |
Regionally restricted |
3E
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Expression in conjunctival epithelium at site where future lacrimal gland will bud.
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| TS22: eye mesenchyme |
Present |
Regionally restricted |
3E
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Expression in mesenchyme adjacent to site of lacrimal gland budding.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Foxc1tm1Blh/Foxc1tm1Blh
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
Present |
Regionally restricted |
3F
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Expression in conjunctival epithelium at site where future lacrimal gland will bud.
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| TS22: eye mesenchyme |
Trace |
Regionally restricted |
3F
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Little or no expression in mesenchyme adjacent to site of lacrimal gland budding.
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Specimen
3G:
embryonic day 13.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
Present |
Regionally restricted |
3G
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Expression in conjunctival epithelium from which future Harderian gland wil bud.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Foxc1tm1Blh/Foxc1tm1Blh
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: conjunctiva epithelium |
Strong |
Regionally restricted |
3H
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Expression appears more intense and domain of expression expanded around developing postoral vibrissa compared to wild type.
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| TS22: face mesenchyme |
Strong |
Regionally restricted |
3H
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Intense expression in condensing mesenchyme on nasal aspect of eye surrounding postoral vibrissa. Higher levels of expression compared to wild type.
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