Gene Expression Data
Assay Details
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Assay
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Reference:
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J:181926
Jo HS, et al., Pten coordinates retinal neurogenesis by regulating Notch signalling. EMBO J. 2012 Feb 15;31(4):817-28
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7764450
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Gene symbol:
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Pou4f2
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Gene name:
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POU domain, class 4, transcription factor 2
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Antibody:
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anti-Brn3b
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
3A Brn3b Pax6-aCre:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Tg(Pax6-cre,GFP)2Pgr/0
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: retina |
retinal ganglion cell |
Present |
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3A
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Specimen
3B Brn3b Pten-cko:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ptentm2Mak/Ptentm2Mak
Tg(Pax6-cre,GFP)2Pgr/0
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: retina |
retinal ganglion cell |
Present |
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3B
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Expression not signficantly different compared to control.
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Specimen
4A Brn3b Pax6-aCre:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Tg(Pax6-cre,GFP)2Pgr/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: retina |
retinal ganglion cell |
Present |
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4A Brn3b Pax6-aCre
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Specimen
4A Brn3b Pten-cko:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Ptentm2Mak/Ptentm2Mak
Tg(Pax6-cre,GFP)2Pgr/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: retina |
retinal ganglion cell |
Present |
Regionally restricted |
4A Brn3b Pten-cko
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Authors report significant increase in numbers of early born cells types (RGCs, ACs and HZs).
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