Chd4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:235368 Gomez-Del Arco P, et al., The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis. Cell Metab. 2016 May 10;23(5):881-92
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7738706
Gene symbol:	Chd4
Gene name:	chromodomain helicase DNA binding protein 4

Antibody:

Anti-Chd4

Results

Specimen 2C WT: postnatal week 8 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	cardiac muscle cell	Present		2C IHC - WT

Specimen 2C Chd4^corin: postnatal week 8; Chd4^tm1.1Kge/Chd4^tm1.1Kge, Corin^tm2(cre)Bamo/Corin^tm2(cre)Bamo (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	cardiac muscle cell	Ambiguous	Regionally restricted	2C IHC - Chd4^corin	Chd4 was deleted in terminally differentiated cardiomyocytes by crossing Chd4<floxed> mice with the Corin-Cre postnatal driver line.

Specimen S1 WT: embryonic day 9.5 (more

)
Note: Double labeled: red - Chd4; green - Tpm.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Present	Regionally restricted	S1A Chd4/Tpm WT	Expression was detected in the left ventricle.
TS15: primitive ventricle endocardial lining	Present	Regionally restricted	S1A Chd4/Tpm WT	Expression was detected in the left ventricle.
TS15: visceral pericardium	Present	Regionally restricted	S1A Chd4/Tpm WT	Expression was detected in the left ventricle.

Specimen S1 Chd4^nkx: embryonic day 9.5; Chd4^tm1.1Kge/Chd4^tm1.1Kge, Nkx2-5^tm2(cre)Rph/Nkx2-5⁺ (more

)
Note: Conditional mutant. Double labeled: red - Chd4; green - Tpm.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle endocardial lining	Present	Regionally restricted	S1A Chd4/Tpm Chd4^nkx	Expression was detected in the left ventricle.
TS15: visceral pericardium	Present	Regionally restricted	S1A Chd4/Tpm Chd4^nkx	Expression was detected in the left ventricle.
TS15: primitive ventricle cardiac muscle	Absent		S1A Chd4/Tpm Chd4^nkx

Specimen S2D WT: postnatal day 21 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	cardiac muscle cell	Present		S2D IHC WT

Specimen S2D Chd4^myh6: postnatal day 21; Chd4^tm1.1Kge/Chd4^tm1.1Kge, Tg(Myh6-cre)2182Mds/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	cardiac muscle cell	Absent		S2D IHC Chd4^myh6	The mutant showed cardiomyocyte-specific ablation.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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