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Gene Expression Data
Assay Details
Assay
Reference: J:83935 Zechner D, et al., beta-Catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system. Dev Biol. 2003 Jun 15;258(2):406-18
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7736712
Gene symbol: Nes
Gene name: nestin
Antibody: anti-nestin
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3a: embryonic day 11.5 (more )
Note: Double labeled: green - Nes; red - Tubb3 (assay MGI:7736711).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord neural progenitor cell Present Regionally restricted 3a
Specimen 3b: embryonic day 11.5; Ctnnb1tm4Wbm/Ctnnb1tm4Wbm, Tg(Pou3f4-cre)32Cren/? (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Tubb3 (assay MGI:7736711).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord neural progenitor cell Present Regionally restricted 3b Reduced area occupied by progenitors compared to controls. Clearly defined progenitor area not apparent ventrally; dorsal progenitor domain still discernable.
Specimen 3c: embryonic day 11.5; Ctnnb1tm1Mmt/Ctnnb1+, Tg(Pou3f4-cre)32Cren/? (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Tubb3 (assay MGI:7736711).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord neural progenitor cell Present Regionally restricted 3c Enlarged progenitor zone compared to control.
Specimen 3d: embryonic day 11.5 (more )
Note: Double labeled: green - Nes; red - Tubb3 (assay MGI:7736711).
Structure Cell Type Level Pattern Image Note
TS19: midbrain neural progenitor cell Present Regionally restricted 3d Expression in precursor cells.
Specimen 3e: embryonic day 11.5; Ctnnb1tm4Wbm/Ctnnb1tm4Wbm, Tg(Pou3f4-cre)32Cren/? (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Tubb3 (assay MGI:7736711).
Structure Cell Type Level Pattern Image Note
TS19: midbrain neural progenitor cell Present Regionally restricted 3e Progenitor domain strongly reduced in size compared to control.
Specimen 3f: embryonic day 11.5; Ctnnb1tm1Mmt/Ctnnb1+, Tg(Pou3f4-cre)32Cren/? (more )
Note: Conditional mutant. Double labeled: green - Nes; red - Tubb3 (assay MGI:7736711).
Structure Cell Type Level Pattern Image Note
TS19: midbrain neural progenitor cell Present Regionally restricted 3f Progenitor domain increased in size compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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