Dio3 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218428 Martinez ME, et al., Genomic imprinting variations in the mouse type 3 deiodinase gene between tissues and brain regions. Mol Endocrinol. 2014 Nov;28(11):1875-86
Assay type:	Northern blot
MGI Accession ID:	MGI:7735065
Gene symbol:	Dio3
Gene name:	deiodinase, iodothyronine type III

Probe:	Dio3 probe
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	4 kb (a)	2.7 kb (b)	2.2 kb (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Dio3 +/+ Neocortex	P7	TS28: neocortex	Not Applicable	Present	Absent	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Not Specified
Dio3 m-/p+ Neocortex	P7	TS28: neocortex	Present	Present	Absent	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3 m+/p- Neocortex	P7	TS28: neocortex	Present	Present	Absent	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Dio3 +/+ T/H/S	P7	TS28: corpus striatum TS28: hippocampus TS28: thalamus	Not Applicable	Present	Present	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Not Specified
Dio3 m-/p+ T/H/S	P7	TS28: corpus striatum TS28: hippocampus TS28: thalamus	Present	Present	Present	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Dio3 m+/p- T/H/S	P7	TS28: corpus striatum TS28: hippocampus TS28: thalamus	Present	Present	Present	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).

Notes:
(a) Mutated transcript. The mutated and the wild-type transcripts appear similarly abundant in Dio3 m-/p+ and Dio3 m+/p- mice. The mutated Dio3 mRNA species have similar levels of expression in neocortex in heterozygous mice, irrespective of the parental origin of the mutated allele. In thalamus/hippocampus/corpus striatum, the pattern of allelic expression in Dio3 m-/p+ and Dio3 m+/p- mice still shows significant preferential expression from the paternally inherited allele.
(b) Wild type transcript. In a P7 specimen comprising the thalamus, hippocampus, and striatum, both 2.2- and 2.7-kb Dio3 transcripts are detected, the latter being more abundant.
(c) Wild type transcript.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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