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Gene Expression Data
Assay Details
Assay
Reference: J:314574 Seo H, et al., Differential expression of hyperpolarization-activated cyclic nucleotide-gated channel subunits during hippocampal development in the mouse. Mol Brain. 2015 Feb 27;8:13
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7716874
Gene symbol: Hcn4
Gene name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Antibody: anti-HCN4 (APC-052)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1G,I: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hippocampus Present Regionally restricted 1G , 1I Expression in hippocampal region, more intense in intermediate zone than in venticular zone. Expression level higher than for Hcn1.
Specimen 2I-1: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: dentate gyrus granule cell layer Present 2I-1 Expression pattern similar to that of Hcn1.
TS27: fimbria hippocampus Present 2I-1 Expression pattern similar to that of Hcn1.
TS27: hippocampus pyramidal cell layer Present 2I-1 Expression pattern similar to that of Hcn1.
TS27: hippocampus stratum lucidum Present 2I-1 Expression pattern similar to that of Hcn1.
TS27: hippocampus stratum oriens Present 2I-1 Expression pattern similar to that of Hcn1.
TS27: hippocampus stratum radiatum Present 2I-1 Expression pattern similar to that of Hcn1.
TS27: hippocampus Present Regionally restricted 2I-1 Expression in cells migrating from ventricular zone, less prominent for Hcn4 than for Hcn1 and Hcn2.
Specimen 2I-2,K,L: postnatal day 7 (more )
Note: Double labeled: red - Hcn4; green - Gfap (assay MGI:7716875).
Structure Cell Type Level Pattern Image Note
TS28: dentate gyrus granule cell layer Present 2I-2 , 2L , 2K
TS28: hippocampus pyramidal cell layer Present 2I-2 , 2L , 2K
TS28: dentate gyrus molecular layer hippocampal astrocyte Present Regionally restricted 2I-2 , 2L , 2K Expression in most astrocytes in stratum lacunosum-moleculare and in border of dentate gyrus molecular layer.
TS28: hippocampus molecular layer hippocampal astrocyte Present Regionally restricted 2I-2 , 2L , 2K Expression in most astrocytes in stratum lacunosum-moleculare and in border of dentate gyrus molecular layer.
TS28: hippocampus stratum lacunosum hippocampal astrocyte Present Regionally restricted 2I-2 , 2L , 2K Expression in most astrocytes in stratum lacunosum-moleculare and in border of dentate gyrus molecular layer.
Specimen 5A,D: postnatal day 21 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA1 Present Regionally restricted 5A , 5D Expression most prominent in stratum lacunosum-moleculare and stratum pyramidale of CA1.
TS28: hippocampus molecular layer Present Regionally restricted 5A , 5D Expression most prominent in stratum lacunosum-moleculare and stratum pyramidale of CA1.
TS28: hippocampus stratum lacunosum Present Regionally restricted 5A , 5D Expression most prominent in stratum lacunosum-moleculare and stratum pyramidale of CA1.
TS28: CA1 pyramidal cell layer Present Regionally restricted 5A , 5D Expression in most Pvalb-expressing cells in stratum pyramidale of CA1.
TS28: fimbria hippocampus Absent 5D , 5A No expression in alveus at this developmental stage.
Specimen 5E,H: postnatal day 21 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present Regionally restricted 5E , 5H Expression most prominent in stratum lacunosum-moleculare and stratum pyramidale of CA2.
TS28: hippocampus molecular layer Present Regionally restricted 5E , 5H Expression most prominent in stratum lacunosum-moleculare and stratum pyramidale of CA2.
TS28: hippocampus stratum lacunosum Present Regionally restricted 5E , 5H Expression most prominent in stratum lacunosum-moleculare and stratum pyramidale of CA2.
TS28: CA2 pyramidal cell layer Present Regionally restricted 5E , 5H Expression in most Pvalb-expressing cells in stratum oriens and stratum pyramidale of CA2.
TS28: hippocampus stratum oriens Present Regionally restricted 5E , 5H Expression in most Pvalb-expressing cells in stratum oriens and stratum pyramidale of CA2.
TS28: fimbria hippocampus Absent 5E , 5H No expression in alveus at this developmental stage.
Specimen 5I,L: postnatal day 21 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA3 Present Regionally restricted 5I , 5L Expression most prominent in stratum lucidum of CA1.
TS28: CA3 pyramidal cell layer Present Regionally restricted 5I , 5L Expression in most Pvalb-expressing cells in stratum ludcidum, stratum pyramidale and stratum oriens of CA3.
TS28: hippocampus stratum lucidum Present Regionally restricted 5I , 5L Expression in most Pvalb-expressing cells in stratum ludcidum, stratum pyramidale and stratum oriens of CA3.
TS28: hippocampus stratum oriens Present Regionally restricted 5I , 5L Expression in most Pvalb-expressing cells in stratum ludcidum, stratum pyramidale and stratum oriens of CA3.
TS28: fimbria hippocampus Absent 5I , 5L No expression in alveus at this developmental stage.
Specimen 5M,P: postnatal day 21 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: dentate gyrus hilus Present Regionally restricted 5M , 5P Expression in most Pvalb-expressing cells in stratum pyramidale of CA1.
Specimen 6I,L: postnatal day 21 (more )
Note: Double labeled: red - Hcn4; green - Dcx (assay MGI:7716877).
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Absent 6I , 6L
Specimen 9A,D: postnatal day 56 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA1 Present Regionally restricted 9A , 9D Expression in most Pvalb-expressing cells in stratum pyramidale of CA1.
TS28: CA1 pyramidal cell layer Present 9D , 9A Expression in stratum lacunosum-moleculare and stratum pyramidale of CA1.
TS28: hippocampus molecular layer Present 9D , 9A Expression in stratum lacunosum-moleculare and stratum pyramidale of CA1.
TS28: hippocampus stratum lacunosum Present 9D , 9A Expression in stratum lacunosum-moleculare and stratum pyramidale of CA1.
TS28: fimbria hippocampus Present Regionally restricted 9A , 9D Expression in alveus of all fields.
Specimen 9E,H: postnatal day 56 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: hippocampus CA2 Present Regionally restricted 9E , 9H Expression in most Pvalb-expressing cells in stratum oriens and stratum radiatum of CA2.
TS28: hippocampus stratum oriens Present Regionally restricted 9E , 9H Expression in most Pvalb-expressing cells in stratum oriens and stratum radiatum of CA2.
TS28: hippocampus stratum radiatum Present Regionally restricted 9E , 9H Expression in most Pvalb-expressing cells in stratum oriens and stratum radiatum of CA2.
TS28: CA2 pyramidal cell layer Present 9H , 9E Expression in stratum lacunosum-moleculare and stratum pyramidale of CA2.
TS28: hippocampus molecular layer Present 9H , 9E Expression in stratum lacunosum-moleculare and stratum pyramidale of CA2.
TS28: hippocampus stratum lacunosum Present 9H , 9E Expression in stratum lacunosum-moleculare and stratum pyramidale of CA2.
TS28: fimbria hippocampus Present Regionally restricted 9E , 9H Expression in alveus of all fields.
Specimen 9I,L: postnatal day 56 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: CA3 pyramidal cell layer Present Regionally restricted 9L , 9I Expression in most Pvalb-expressing cells in stratum lacunosum and stratum pyramidale of CA3.
TS28: hippocampus CA3 Present Regionally restricted 9L , 9I Expression in most Pvalb-expressing cells in stratum lacunosum and stratum pyramidale of CA3.
TS28: hippocampus molecular layer Present Regionally restricted 9L , 9I Expression in most Pvalb-expressing cells in stratum lacunosum and stratum pyramidale of CA3.
TS28: hippocampus stratum lacunosum Present Regionally restricted 9L , 9I Expression in most Pvalb-expressing cells in stratum lacunosum and stratum pyramidale of CA3.
TS28: fimbria hippocampus Present Regionally restricted 9L , 9I Expression in alveus of all fields.
Specimen 9M,P: postnatal day 56 (more )
Note: Double labeled: red - Hcn4; green - Pvalb (assay MGI:7716876).
Structure Level Pattern Image Note
TS28: dentate gyrus hilus Present Regionally restricted 9P , 9M Expression in most Pvalb-expressing cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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