Antibody:	anti-HCN2 (APC-030)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Regionally restricted	1F , 1D	Expression in hippocampal region, more intense in intermediate zone than in venticular zone. Expression level higher than for Hcn1.

Structure	Level	Pattern	Image	Note
TS27: fimbria hippocampus	Present	Regionally restricted	2E-1	Expression in alveus.
TS27: dentate gyrus granule cell layer	Present		2E-1
TS27: hippocampus pyramidal cell layer	Present		2E-1
TS27: hippocampus stratum lucidum	Present		2E-1
TS27: hippocampus stratum radiatum	Present		2E-1
TS27: hippocampus	Present	Regionally restricted	2E-1	Expression in cells migrating from ventricular zone, more prominent for Hcn1 and Hcn2 than for Hcn4.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer		Present		2E-2 , 2H , 2G
TS28: hippocampus molecular layer		Present		2E-2 , 2H , 2G
TS28: hippocampus pyramidal cell layer		Present		2E-2 , 2H , 2G
TS28: hippocampus stratum lacunosum		Present		2E-2 , 2H , 2G
TS28: hippocampus	hippocampal neuron	Present	Regionally restricted	2G , 2H , 2E-2	Expression in neuronal somata.
TS28: hippocampus	hippocampal astrocyte	Absent		2G , 2E-2 , 2H	No expression in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present	Regionally restricted	4D , 4A	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale.
TS28: hippocampus molecular layer	Present	Regionally restricted	4D , 4A	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale.
TS28: hippocampus stratum lacunosum	Present	Regionally restricted	4D , 4A	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale.
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	4D , 4A	Expression in most Pvalb-expressing cells.
TS28: fimbria hippocampus	Absent		4D , 4A	No expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present	Regionally restricted	4H , 4E	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale, particularly in CA3. Stronger expression than in CA1 and CA2.
TS28: hippocampus molecular layer	Present	Regionally restricted	4H , 4E	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale, particularly in CA3. Stronger expression than in CA1 and CA2.
TS28: hippocampus stratum lacunosum	Present	Regionally restricted	4H , 4E	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale, particularly in CA3. Stronger expression than in CA1 and CA2.
TS28: CA3 pyramidal cell layer	Present	Regionally restricted	4E , 4H	Expression in most Pvalb-expressing cells.
TS28: fimbria hippocampus	Absent		4E , 4H	No expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present	Regionally restricted	4L , 4I	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale, particularly in CA3. Stronger expression than in CA1 and CA2.
TS28: hippocampus molecular layer	Present	Regionally restricted	4L , 4I	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale, particularly in CA3. Stronger expression than in CA1 and CA2.
TS28: hippocampus stratum lacunosum	Present	Regionally restricted	4L , 4I	Expression more prominent in stratum lacunosum-moleculare and stratum pyramidale, particularly in CA3. Stronger expression than in CA1 and CA2.
TS28: CA2 pyramidal cell layer	Present	Regionally restricted	4L , 4I	Expression in most Pvalb-expressing cells.
TS28: fimbria hippocampus	Absent		4L , 4I	No expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus hilus	Absent		4M , 4P	No expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Absent		6E , 6H

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present	Regionally restricted	8A , 8D	Expression in most Pvalb-expressing cells.
TS28: hippocampus molecular layer	Present		8D , 8A	Expression most prominent in stratum lacunosum-moleculare of CA1.
TS28: hippocampus stratum lacunosum	Present		8D , 8A	Expression most prominent in stratum lacunosum-moleculare of CA1.
TS28: fimbria hippocampus	Present	Regionally restricted	8D , 8A	Expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present	Regionally restricted	8E , 8H	Expression in most Pvalb-expressing cells.
TS28: CA2 pyramidal cell layer	Present		8H , 8E	Expression most prominent in stratum pyramidale of CA2.
TS28: fimbria hippocampus	Present	Regionally restricted	8E , 8H	Expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Present	Regionally restricted	8I , 8L	Expression in most Pvalb-expressing cells.
TS28: CA3 pyramidal cell layer	Present		8I , 8L	Expression most prominent in stratum pyramidale of CA3.
TS28: fimbria hippocampus	Present	Regionally restricted	8L , 8I	Expression in alveus at this developmental stage.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	8P , 8M	Expression in most Pvalb-expressing cells.