Gene Expression Data
Assay Details
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Assay
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Reference:
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J:154889
Deschamps C, et al., Expression of ephrinA5 during development and potential involvement in the guidance of the mesostriatal pathway. Exp Neurol. 2009 Oct;219(2):466-80
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7714977
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4a:
embryonic day 12.5
(more  )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4a:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
4a
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Positive neuron somas were located in the ventral mesencephalon.
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Specimen
4b:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4b
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Axons grew in the diencephalon ventrally to the thalamus to reach the caudal region of the lateral ganglionic eminence.
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Specimen
4c:
embryonic day 12.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4c:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4c
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Axons grew in the diencephalon ventrally to the thalamus to reach the caudal region of the lateral ganglionic eminence.
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| TS20: lateral ganglionic eminence |
Absent |
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4c
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Positive projections were detected neither ventrally of the lateral ganglionic eminence nor inside this structure.
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Specimen
4d:
embryonic day 14.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4d:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
4d
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Positive neuron somas were located in the ventral mesencephalon.
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Specimen
4e:
embryonic day 14.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4e:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4e
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Axons grew in the diencephalon ventrally to the thalamus to reach the caudal region of the lateral ganglionic eminence.
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Specimen
4f:
embryonic day 14.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4f:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
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Specimen
4g:
embryonic day 16.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4g:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
4g
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Positive neuron somas were located in the ventral mesencephalon.
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Specimen
4h:
embryonic day 16.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4h:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: diencephalon |
Present |
Regionally restricted |
4h
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Axons grew in the diencephalon ventrally to the thalamus to reach the caudal region of the lateral ganglionic eminence.
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Specimen
4i:
embryonic day 16.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4i:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
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Specimen
4j:
embryonic day 18.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4j:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
4j
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Positive neuron somas were located in the ventral mesencephalon.
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Specimen
4k:
embryonic day 18.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4k:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
|
Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: diencephalon |
Present |
Regionally restricted |
4k
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Axons grew in the diencephalon ventrally to the thalamus to reach the caudal region of the lateral ganglionic eminence.
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Specimen
4l:
embryonic day 18.5
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
4l:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: corpus striatum |
Present |
Regionally restricted |
4l
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A distinct ventro-dorsal distribution of the TH+ projections mainly concentrated in the ventral part.
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| TS26: ventral striatum |
Present |
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4l
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Specimen
5a:
postnatal day 0
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5a
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Positive neuron somas were located in the ventral mesencephalon.
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Specimen
5b:
postnatal day 0
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: diencephalon |
Present |
Regionally restricted |
5b
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The positive medial forebrain bundle was seen in the ventral region.
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Specimen
5c:
postnatal day 0
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5c:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: corpus striatum |
Present |
|
5c
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TH+ axons were clearly distributed towards the dorsal part.
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Specimen
5d:
postnatal day 7
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5d:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5d
|
Positive neuron somas were located in the ventral mesencephalon.
|
Specimen
5e:
postnatal day 7
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5e:
(close )
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Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: diencephalon |
Present |
Regionally restricted |
5e
|
The positive medial forebrain bundle was seen in the ventral region.
|
Specimen
5f:
postnatal day 7
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5f:
(close )
|
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus striatum |
Present |
Widespread |
5f
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TH+ axons were connected to the entire striatum.
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Specimen
5g:
postnatal month 4
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5g:
(close )
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Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal month 4
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Sex:
|
Not Specified
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|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
5g
|
Positive neuron somas were located in the ventral mesencephalon.
|
Specimen
5h:
postnatal month 4
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5h:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: diencephalon |
Present |
Regionally restricted |
5h
|
The positive medial forebrain bundle was seen in the ventral region.
|
Specimen
5i:
postnatal month 4
(more )
Note: Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
Specimen
5i:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: brown - Th; blue - Efna5 (assay MGI:7714978).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus striatum |
Present |
Widespread |
5i
|
TH+ axons were connected to the entire striatum.
|
Specimen
11a:
postnatal month 4
(more )
Specimen
11a:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
11a
|
Positive neuron somas were located in the ventral mesencephalon.
|
Specimen
11b:
postnatal month 4
(more )
Specimen
11b:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: diencephalon |
Present |
Regionally restricted |
11b
|
Expressed in medial forebrain bundle.
|
Specimen
11c:
postnatal month 4
(more )
Specimen
11c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
11d:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Efna5tm1Ddmo/Efna5tm1Ddmo
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
11d
|
Positive neuron somas were located in the ventral mesencephalon, although weaker with similar patterns as in the wild-type.
|
Specimen
11e:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Efna5tm1Ddmo/Efna5tm1Ddmo
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: diencephalon |
Present |
Regionally restricted |
11e
|
Expressed in medial forebrain bundle, although weaker with similar patterns as in the wild-type.
|
Specimen
11f:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Efna5tm1Ddmo/Efna5tm1Ddmo
|
|
Age:
|
postnatal month 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus striatum |
Present |
|
11f
|
Expressed in striatal projections, although weaker with similar patterns as in the wild-type.
|
|
Analysis Tools