Nefl Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:348858 Zaninello M, et al., CLUH maintains functional mitochondria and translation in motoneuronal axons and prevents peripheral neuropathy. Sci Adv. 2024 May 31;10(22):eadn2050
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7714919
Gene symbol:	Nefl
Gene name:	neurofilament, light polypeptide

Antibody:	anti-Neurofilament-L (C28E10) (#2837)
Detection system:	Secondary antibody coupled to Alexa Fluor 647

Results

3C WT ©

Specimen 3C WT: postnatal month 14; Cluh^tm1.1Eir/Cluh^tm1.1Eir (more

)
Note: Vibratome 250um section. Double labeled: white - Nefl; green - alpha-Bungarotoxin.

Structure	Level	Pattern	Image	Note
TS28: deep peroneal nerve	Present		3C WT

3C NKO ©

Specimen 3C NKO: postnatal month 14; Cluh^tm1.1Eir/Cluh^tm1.1Eir, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Vibratome 250um section. Double labeled: white - Nefl; green - alpha-Bungarotoxin.

Structure	Level	Pattern	Image	Note
TS28: deep peroneal nerve	Present		3C NKO	Increased number of denervated or poorly innervated neuromuscular junctions.

3E WT ©

Specimen 3E WT: postnatal month 14; Cluh^tm1.1Eir/Cluh^tm1.1Eir (more

)
Note: Vibratome 250um section. Double labeled: white - Nefl; green - alpha-Bungarotoxin.

Structure	Level	Pattern	Image	Note
TS28: deep peroneal nerve	Present		3E WT

3E NKO ©

Specimen 3E NKO: postnatal month 14; Cluh^tm1.1Eir/Cluh^tm1.1Eir, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant. Vibratome 250um section. Double labeled: white - Nefl; green - alpha-Bungarotoxin.

Structure	Level	Pattern	Image	Note
TS28: deep peroneal nerve	Present		3E NKO	Denervated or poorly innervated neuromuscular junctions were shown.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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