Gene Expression Data
Assay Details
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Assay
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Reference:
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J:269981
Marcucci F, et al., Distinct timing of neurogenesis of ipsilateral and contralateral retinal ganglion cells. J Comp Neurol. 2019 Jan 1;527(1):212-224
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7708047
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Gene symbol:
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Mki67
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Gene name:
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antigen identified by monoclonal antibody Ki 67
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Results
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Specimen
4a:
embryonic day 14.5
(more  )
Note: Triple labeled: red - Mki67; blue - Isl1 (assay MGI:7708046); green - EdU. EdU injected at E13.
Specimen
4a:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Mki67; blue - Isl1 (assay MGI:7708046); green - EdU. EdU injected at E13.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
4a
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Expression was detected in proliferating cells. Within the ventral retina, there was a significant decrease in the percentage of progenitors (% EdU+ Ki67-/EdU+) that exit cell cycle in the ventral peripheral neural retina and the ciliary margin zone, when compared with the ventral central neural retina.
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Specimen
4b:
embryonic day 14.5
(more )
Note: Triple labeled: red - Mki67; blue - Isl1 (assay MGI:7708046); green - EdU. EdU injected at E13.
Specimen
4b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Mki67; blue - Isl1 (assay MGI:7708046); green - EdU. EdU injected at E13.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
4b
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Expression was detected in proliferating cells. In the dorsal peripheral zone the number of cells exiting cycle (% EdU+ Ki67-/EdU+) in the dorsal ciliary margin zone and peripheral neural retina was comparable to that in the dorsal central zone.
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Specimen
not shown Ccnd2+/+:
embryonic day 14.5
(more )
Note: EdU injected at E13.
Specimen
not shown Ccnd2+/+:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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EdU injected at E13.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
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Expression was detected in proliferating cells.
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Specimen
not shown Ccnd2-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ccnd2tm1Wbg/Ccnd2tm1Wbg
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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EdU injected at E13.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
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Expression was detected in proliferating cells. Cell exit (% EdU+ Ki67-/EdU+) was reduced in both ventral and dorsal ciliary margin zone of Cyclin D2-/- mice compared with cell cycle exit in wild type. However, cell cycle exit was similar within the peripheral retina of wild type and Cyclin D2-/- mice.
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