Antibody:	Anti-GnRH/LHRH (20075)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		2D	Immunolabeled migrating GnRH neurons were identified.
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present		2D	Immunolabeled migrating GnRH neurons were identified.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		2E	Immunolabeled migrating GnRH neurons followed a normal path in the mutant.
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present		2E	Immunolabeled migrating GnRH neurons followed a normal path in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial preoptic area	hypothalamic gonadotropin-releasing hormone neuron	Present		2G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial preoptic area	hypothalamic gonadotropin-releasing hormone neuron	Present		2H	Immunolabeled GnRH neurons were detected in normal numbers in the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		3A	Immunolabeled migrating GnRH neurons were identified.
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present		3A	Immunolabeled migrating GnRH neurons were identified.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present		3B	Immunolabeled migrating GnRH neurons were identified.
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present	Single cells	3B	Few GnRH neurons were detected in the forebrain.
TS22: nasal mesenchyme	hypothalamic gonadotropin-releasing hormone neuron	Present	Regionally restricted	3B	Ectopic GnRH neurons were detected in the nasal mesenchyme at the level of the cribriform plate.
TS22: head	hypothalamic gonadotropin-releasing hormone neuron	Present	Regionally restricted	3B , 3B'	The majority of immunolabeled migrating GnRH neurons did not enter the forebrain in the mutant. Ectopic neurons were identified in the tissue distal to the normal forebrain entry point, they were outside the brain and adjacent to the meninges. Cell counts indicated the mutant contained a normal number of GnRH neurons overall.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		3G

Structure	Cell Type	Level	Pattern	Image	Note
TS26: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present	Single cells	3H	The number of GnRH neurons was severely reduced in the mutant compared to wild type.

Structure	Level	Pattern	Image	Note
TS26: median eminence	Present	Regionally restricted	3I	GnRH neuron axonal projections were labeled in the median eminence.

Structure	Level	Pattern	Image	Note
TS26: median eminence	Absent		3J

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hypothalamus	hypothalamic gonadotropin-releasing hormone neuron	Present		3K
TS28: median eminence		Present	Regionally restricted	3K	GnRH neuron axonal projections were labeled in the median eminence.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hypothalamus	hypothalamic gonadotropin-releasing hormone neuron	Present	Single cells	3L	The number of GnRH neurons was severely reduced in the mutant compared to wild type.
TS28: median eminence		Weak	Regionally restricted	3L	GnRH neuron projections to the median eminence were markedly reduced in adult Sema3a-null males compared with wild-type littermates.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		5A	Immunolabeled migrating GnRH neurons were identified.
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present		5A	Immunolabeled migrating GnRH neurons were identified.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		5B	Immunolabeled migrating GnRH neurons were identified.
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present		5B	Immunolabeled migrating GnRH neurons were identified.
TS22: head	hypothalamic gonadotropin-releasing hormone neuron	Present	Regionally restricted	5B	A few GnRH neurons migrated ectopically towards the meninges.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		5C	Immunolabeled migrating GnRH neurons were identified.
TS22: nose	hypothalamic gonadotropin-releasing hormone neuron	Present	Regionally restricted	5C	Small aggregates of GnRH neurons were observed in the nasal comparment of the mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		5D	Immunolabeled migrating GnRH neurons were identified.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: head	hypothalamic gonadotropin-releasing hormone neuron	Present	Regionally restricted	5E	Most GnRH neurons failed to enter the forebrain, but, instead, accumulated at the level of the cribriform plate or took an ectopic path towards the meninges.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: forebrain	hypothalamic gonadotropin-releasing hormone neuron	Present		S3