Results
|
Specimen
1A Ctrl:
embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Quantification was shown in the 1A right graphs.
Specimen
1A Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 1A right graphs.
|
Specimen
1A cKO:
embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Conditional mutant. Quantification was shown in the 1A right graphs.
Specimen
1A cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1A right graphs.
|
Specimen
1A cKO;p53-/-:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S2/SvPas
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+ Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1A right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Strong |
|
1A right
|
A high level of DNA damage throughout the cortex was seen, higher than the single cKO.
|
Specimen
not shown Ctrl:
embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Quantification was shown in the 1A right graphs.
Specimen
not shown Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 1A right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Present |
Regionally restricted |
|
Only a few foci were seen in hippocampal hem (future hippocampus) and basal ganglia.
|
Specimen
not shown cKO:
embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Conditional mutant. Quantification was shown in the 1A right graphs.
Specimen
not shown cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1A right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Strong |
Regionally restricted |
|
A high level of DNA damage throughout the hippocampal hem (future hippocampus) and basal ganglia was seen.
|
Specimen
not shown cKO;p53-/-:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S2/SvPas
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+ Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1A right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Strong |
Regionally restricted |
|
A high level of DNA damage throughout the hippocampal hem (future hippocampus) and basal ganglia was seen. Expression in the hippocampal hem was higher than the single cKO.
|
Specimen
not shown p53-/-:
embryonic day 13.5; Trp53tm1Tyj/ Trp53tm1Tyj
(more )
Note: Quantification was shown in the 1A right graphs.
Specimen
not shown p53-/-:
(close )
Genetic Background:
|
involves: 129S2/SvPas
|
Mutant Allele(s):
|
Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 1A right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
|
Similar as in the control.
|
TS21: telencephalon |
Present |
Regionally restricted |
|
Expression in the hippocampal hem (future hippocampus) and basal ganglia was similar as in the control.
|
Specimen
1B Ctrl:
postnatal day 0.5; Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Quantification was shown in the 1B right graphs.
Specimen
1B Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 1B right graphs.
|
Specimen
1B cKO:
postnatal day 0.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Conditional mutant. Quantification was shown in the 1B right graphs.
Specimen
1B cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1B right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebral cortex |
Present |
|
1B middle
|
The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the cortex was seen.
|
Specimen
1B cKO;p53-/-:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S2/SvPas
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+ Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1B right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebral cortex |
Present |
|
1B right
|
The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the cortex was seen.
|
Specimen
not shown Ctrl:
postnatal day 0.5; Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Quantification was shown in the 1B right graphs.
Specimen
not shown Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 1B right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: hippocampus |
Present |
|
|
Only a few foci were seen.
|
Specimen
not shown cKO:
postnatal day 0.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Conditional mutant. Quantification was shown in the 1B right graphs.
Specimen
not shown cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1B right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: hippocampus |
Present |
|
|
The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the cortex was seen.
|
Specimen
not shown cKO;p53-/-:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S2/SvPas
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+ Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 1B right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: hippocampus |
Present |
|
|
The level of DNA damage was appreciably lower in the neonatal brain. A higher level of DNA damage throughout the hippocampus was seen, higher than the single cKO.
|
Specimen
not shown p53-/-:
postnatal day 0.5; Trp53tm1Tyj/ Trp53tm1Tyj
(more )
Note: Quantification was shown in the 1B right graphs.
Specimen
not shown p53-/-:
(close )
Genetic Background:
|
involves: 129S2/SvPas
|
Mutant Allele(s):
|
Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
postnatal day 0.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 1B right graphs.
|
Specimen
1C Ctrl:
embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
Specimen
1C Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
|
Specimen
1C cKO:
embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
Specimen
1C cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Present |
|
1C cKO right
,
1C cKO left
|
The majority of cells undergoing apoptosis (i.e., cleaved caspase-3+ cells) in the cKO telencephalon also stained positive for H2ax.
|
Specimen
not shown cKO;p53-/-:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * 129S2/SvPas
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+ Trp53tm1Tyj/Trp53tm1Tyj
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: red - H2ax; green - Casp3 (assay MGI:7705315). Quantification was shown in the 1C right graphs.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Trace |
|
|
Apoptosis (i.e., cleaved caspase-3+ cells) was rare in the cortex, hippocampal hem (future hippocampus) and basal ganglia. The majority of cells undergoing apoptosis in the cortex and basal ganglia also stained positive for H2ax.
|
Specimen
3C Ctrl:
embryonic day 13.5; Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Quantification was shown in the 3C right graphs. Double labeled: red - H2ax; green - Pcna (assay MGI:7705325).
Specimen
3C Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Quantification was shown in the 3C right graphs. Double labeled: red - H2ax; green - Pcna (assay MGI:7705325).
|
Specimen
3C cKO:
embryonic day 13.5; Atrxtm1Rjg/Y, Foxg1tm1(cre)Skm/Foxg1 +
(more )
Note: Conditional mutant. Quantification was shown in the 3C right graphs. Double labeled: red - H2ax; green - Pcna (assay MGI:7705325).
Specimen
3C cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Quantification was shown in the 3C right graphs. Double labeled: red - H2ax; green - Pcna (assay MGI:7705325).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
3C cKO merge
,
3C cKO gammaH2AX
|
More DNA damage (H2ax+) at the late replication foci (Pcna+) compared with control cells, confirming an increase in double-stranded breaks during the replication process.
|
Specimen
7E Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
7E cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant.
|
Specimen
S1A Ctrl:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
postnatal day 7
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
S1A cKO:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Atrxtm1Rjg/Y Foxg1tm1(cre)Skm/Foxg1+
|
Age:
|
postnatal day 7
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant.
|
|