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Gene Expression Data
Assay Details
Assay
Reference: J:93103 Diaz D, et al., Effect of methylmercury on glutamate-cysteine ligase expression in the placenta and yolk sac during mouse development. Reprod Toxicol. 2004 Nov;19(1):117-29
Assay type: RT-PCR
MGI Accession ID: MGI:7664462
Gene symbol: Gclm
Gene name: glutamate-cysteine ligase, modifier subunit
Probe: Gclm-pF, Gclm-pR
Probe preparation: labelled with FAM
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh and expressed as % of gd 10 expression levels.
Results Image: 2B
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
10 gd E10.5 (a) TS17: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
12 gd E12.5 (a) TS20: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
14.a E14.5 (a) TS22: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
14.b E14.5 (a) TS22: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
14.c E14.5 (a) TS22: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
14.d E14.5 (a) TS22: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
16 gd E16.5 (a) TS24: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
18.a E18.5 (a) TS26: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
18.b E18.5 (a) TS26: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
18.c E18.5 (a) TS26: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
18.d E18.5 (a) TS26: yolk sac Present 2 µg; total RNA C57BL/6 Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression levels were similar throughout development.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory