Gene Expression Data
Assay Details
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Assay
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Reference:
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J:336866
Puelles L, et al., The Midbrain Preisthmus: A Poorly Known Effect of the Isthmic Organizer. Int J Mol Sci. 2023 Jun 5;24(11)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7664443
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Gene symbol:
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Tal1
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Gene name:
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T cell acute lymphocytic leukemia 1
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Probe:
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Tal1_riboprobe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors analyzed data from the Allen Developing Mouse Brain.
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Results
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Specimen
3m:
embryonic day 11.5
(more  )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
3m:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain mantle layer |
Strong |
Regionally restricted |
3m
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Strong expression was detected in the midbrain basal domain, but no staining was observed in the caudal sulcal area.
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| TS19: midbrain ventricular layer |
Strong |
Regionally restricted |
3m
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Strong expression was detected in the midbrain basal domain, but no staining was observed in the caudal sulcal area.
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Specimen
5p:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5p:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
5p
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Marked expression was detected laterally at the alar preisthmus.
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Specimen
5q:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5q:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
5q
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Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
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| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5q
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A basal signal was detected in the isthmus.
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Specimen
5r:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5r:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
5r
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Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
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| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5r
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A basal signal was detected in the isthmus.
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Specimen
5s:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5s:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
5s
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Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
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| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5s
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A basal signal was detected in the isthmus.
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Specimen
5t:
embryonic day 13.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
5t:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: mesomere 2 |
Present |
Regionally restricted |
5t
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Expression was detected more ventrally along the transition into the preisthmic basal domain, where it is separated distinctly from an analogous isthmic basal signal by a negative narrow tissue gap associated with the posterior intraencephalic sulcus.
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| TS22: midbrain-hindbrain junction |
Present |
Regionally restricted |
5t
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A basal signal was detected in the isthmus.
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Specimen
7t:
embryonic day 15.5
(more )
Note: Sample was postfixed in 4% paraformaldehyde.
Specimen
7t:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Sample was postfixed in 4% paraformaldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: mesomere 2 |
Present |
Regionally restricted |
7t
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Expression was detected in the preisthmus and showed a curved boundary with the isthmus, with development of a marked convexity towards the midbrain.
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