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Gene Expression Data
Assay Details
Assay
Reference: J:232771 Messerschmidt D, et al., beta-catenin-mediated adhesion is required for successful preimplantation mouse embryo development. Development. 2016 Jun 1;143(11):1993-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7664159
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4 (C-10; sc-5279)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2F delta/+: embryonic day 3.5; Ctnnb1tm1Knw/Ctnnb1+, Tg(Zp3-cre)93Knw/0 (more )
Note: Conditional mutant. Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7664156).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2F delta/+ Merge , 2F delta/+ OCT4
Specimen 2F delta/delta: embryonic day 3.5; Ctnnb1tm1Knw/Ctnnb1tm1Knw, Tg(Zp3-cre)93Knw/0 (more )
Note: Conditional mutant. Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7664156).
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 2F delta/delta OCT4 , 2F delta/delta Merge Expression in all cells of the embryo.
Specimen S4B: embryonic day 4.5; Ctnnb1tm1Knw/Ctnnb1+, Tg(Zp3-cre)93Knw/0 (more )
Note: Conditional mutant. Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7664156).
Structure Level Pattern Image Note
TS6: epiblast Present S4B OCT4 , S4B MERGE
TS6: primitive endoderm Present S4B OCT4 , S4B MERGE
Specimen S4A: embryonic day 4.5; Ctnnb1tm1Knw/Ctnnb1tm1Knw, Tg(Zp3-cre)93Knw/0 (more )
Note: Conditional mutant. Double labeled: red - Pou5f1; green - Ctnnb1 (assay MGI:7664156).
Structure Level Pattern Image Note
TS6: epiblast Present S4A MERGE , S4A OCT4 Expression confined to pluripotent epiblast and primitive endoderm.
TS6: primitive endoderm Present S4A MERGE , S4A OCT4 Expression confined to pluripotent epiblast and primitive endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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