Ctnnb1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:232771 Messerschmidt D, et al., beta-catenin-mediated adhesion is required for successful preimplantation mouse embryo development. Development. 2016 Jun 1;143(11):1993-9
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7664156
Gene symbol:	Ctnnb1
Gene name:	catenin beta 1

Antibody:	anti-beta-catenin (#9581)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

2B delta/+ Merge ©

2B delta/+ n-term CTNNB1 ©

Specimen 2B delta/+: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1⁺, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1 N-terminal; red - Ctnnb1 C-terminal (assay MGI:7664157).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2B delta/+ n-term CTNNB1 , 2B delta/+ Merge

2B delta/delta Merge ©

2B delta/delta n-term CTNNB1 ©

Specimen 2B delta/delta: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1^tm1Knw, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1 N-terminal; red - Ctnnb1 C-terminal (assay MGI:7664157).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		2B delta/delta Merge , 2B delta/delta n-term CTNNB1

2E delta/+ Merge ©

2E delta/+ n-term CTNNB1 ©

Specimen 2E delta/+: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1⁺, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Casp3 (assay MGI:7664158).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2E delta/+ n-term CTNNB1 , 2E delta/+ Merge

2E delta/delta Merge ©

2E delta/delta n-term CTNNB1 ©

Specimen 2E delta/delta: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1^tm1Knw, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Casp3 (assay MGI:7664158).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		2E delta/delta Merge , 2E delta/delta n-term CTNNB1

2F delta/+ Merge ©

2F delta/+ n-term CTNNB1 ©

Specimen 2F delta/+: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1⁺, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Pou5f1 (assay MGI:7664159).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2F delta/+ n-term CTNNB1 , 2F delta/+ Merge

2F delta/delta Merge ©

2F delta/delta n-term CTNNB1 ©

Specimen 2F delta/delta: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1^tm1Knw, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Pou5f1 (assay MGI:7664159).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		2F delta/delta n-term CTNNB1 , 2F delta/delta Merge

2G delta/+ Merge ©

2G delta/+ n-term CTNNB1 ©

Specimen 2G delta/+: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1⁺, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Cdx2 (assay MGI:7664160).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2G delta/+ Merge , 2G delta/+ n-term CTNNB1

2G delta/delta Merge ©

2G delta/delta n-term CTNNB1 ©

Specimen 2G delta/delta: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1^tm1Knw, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Cdx2 (assay MGI:7664160).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		2G delta/delta Merge , 2G delta/delta n-term CTNNB1

Specimen 2H delta/+: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1⁺, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Sox17 (assay MGI:7664161).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		2H delta/+ n-term CTNNB1 , 2H delta/+ Merge

Specimen 2H delta/delta: embryonic day 3.5; Ctnnb1^tm1Knw/Ctnnb1^tm1Knw, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Sox17 (assay MGI:7664161).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent		2H delta/delta n-term CTNNB1 , 2H delta/delta Merge

S4B MERGE ©

Specimen S4B: embryonic day 4.5; Ctnnb1^tm1Knw/Ctnnb1⁺, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Pou5f1 (assay MGI:7664159).

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present		S4B MERGE , S4B n-term CTNNB1

S4A MERGE ©

Specimen S4A: embryonic day 4.5; Ctnnb1^tm1Knw/Ctnnb1^tm1Knw, Tg(Zp3-cre)93Knw/0 (more

)
Note: Conditional mutant. Double labeled: green - Ctnnb1; red - Pou5f1 (assay MGI:7664159).

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Absent		S4A MERGE , S4A n-term CTNNB1

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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