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Gene Expression Data
Assay Details
Assay
Reference: J:305426 Du G, et al., Proper timing of a quiescence period in precursor prospermatogonia is required for stem cell pool establishment in the male germline. Development. 2021 May 1;148(9):dev194571
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7662973
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody
Results
Specimen 3F Control E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS22: germ cell of testis Present 3F Control E14.5
Specimen 3F Control E16.5: embryonic day 16.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Single cells 3F Control E16.5
Specimen 3F Control E18.5: embryonic day 18.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3F Control E18.5
Specimen 3F Rb1-cKOBlimp1 E14.5: embryonic day 14.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS22: germ cell of testis Present 3F Rb1-cKOBlimp1 E14.5 The level of germ cell apoptosis was the same between control and this mutant.
Specimen 3F Rb1-cKOBlimp1 E16.5: embryonic day 16.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS24: germ cell of testis Present Single cells 3F Rb1-cKOBlimp1 E16.5 About 44.2% of prospermatogonia were apoptotic in this mutant compared with the 6.9% in control mice.
Specimen 3F Rb1-cKOBlimp1 E18.5: embryonic day 18.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3F Rb1-cKOBlimp1 E18.5 The level of germ cell apoptosis in this mutant had increased compared to controls.
Specimen S2C Control: embryonic day 15.5 (more )
Note: Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S2C Control Cleaved CASPASE-3 , S2C Control Merge
Specimen S2C Rb1-cKOBlimp1: embryonic day 15.5; Rb1tm2Brn/Rb1tm2Brn, Tg(Prdm1-cre)1Masu/0 (more )
Note: Conditional mutant. Double labeled: green - Casp3; red - Gcna (assay MGI:7662958).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S2C Rb1-cKOBlimp1 Merge , S2C Rb1-cKOBlimp1 Cleaved CASPASE-1 The level of germ cell apoptosis was the same between control and this mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory