Gcna Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:305426 Du G, et al., Proper timing of a quiescence period in precursor prospermatogonia is required for stem cell pool establishment in the male germline. Development. 2021 May 1;148(9):dev194571
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7662958
Gene symbol:	Gcna
Gene name:	germ cell nuclear acidic protein

Antibody:	TRA98 (ab82527)
Detection system:	Secondary antibody

Results

1_Ki67 E12.5 ©

Specimen 1 E12.5 Ki67: embryonic day 12.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS20: germ cell of testis	Present		1_Ki67 E12.5

1_Ki67 E13.5 ©

Specimen 1 E13.5 Ki67: embryonic day 13.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		1_Ki67 E13.5

1_Ki67 E14.5 ©

Specimen 1 E14.5 Ki67: embryonic day 14.4 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		1_Ki67 E14.5

1_Ki67 E16.5 ©

Specimen 1 E16.5 Ki67: embryonic day 16.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		1_Ki67 E16.5

1_Ki67 P1.5 ©

Specimen 1 P1.5 Ki67: postnatal day 1.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		1_Ki67 P1.5

1_Ki67 P6.5 ©

Specimen 1 P6.5 Ki67: postnatal day 6.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		1_Ki67 P6.5

1_RB1 E12.5 ©

Specimen 1 E12.5 RB1: embryonic day 12.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662968).

Structure	Level	Pattern	Image	Note
TS20: germ cell of testis	Present		1_RB1 E12.5

1_RB1 E13.5 ©

Specimen 1 E13.5 RB1: embryonic day 13.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662968).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		1_RB1 E13.5

1_RB1 E14.5 ©

Specimen 1 E14.5 RB1: embryonic day 14.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662968).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		1_RB1 E14.5

1_RB1 E16.5 ©

Specimen 1 E16.5 RB1: embryonic day 16.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662968).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		1_RB1 E16.5

1_RB1 P1.5 ©

Specimen 1 P1.5 RB1: postnatal day 1.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662968).

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		1_RB1 P1.5

1_RB1 P6.5 ©

Specimen 1 P6.5 RB1: postnatal day 6.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662968).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		1_RB1 P6.5

1_p-RB1 E12.5 ©

Specimen 1 E12.5 p-RB1: embryonic day 12.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662969).

Structure	Level	Pattern	Image	Note
TS20: germ cell of testis	Present		1_p-RB1 E12.5

1_p-RB1 E13.5 ©

Specimen 1 E13.5 p-RB1: embryonic day 13.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662969).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		1_p-RB1 E13.5

1_p-RB1 E14.5 ©

Specimen 1 E14.5 p-RB1: embryonic day 14.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662969).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		1_p-RB1 E14.5

1_p-RB1 E16.5 ©

Specimen 1 E16.5 p-RB1: embryonic day 16.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662969).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		1_p-RB1 E16.5

1_p-RB1 P1.5 ©

Specimen 1 P1.5 p-RB1: postnatal day 1.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662969).

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		1_p-RB1 P1.5

1_p-RB1 P6.5 ©

Specimen 1 P6.5 p-RB1: postnatal day 6.5 (more

)
Note: Double labeled: red - Gcna; green - Rb1 (assay MGI:7662969).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		1_p-RB1 P6.5

3A Control E14.5 ©

Specimen 3A Control E14.5: embryonic day 14.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3A Control E14.5

3A Control E16.5 ©

Specimen 3A Control E16.5: embryonic day 16.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3A Control E16.5

3A Control E18.5 ©

Specimen 3A Control E18.5: embryonic day 18.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		3A Control E18.5

3A Rb1-cKO^Blimp1 E14.5 ©

Specimen 3A Rb1-cKO^Blimp1 E14.5: embryonic day 14.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3A Rb1-cKO^Blimp1 E14.5

3A Rb1-cKO^Blimp1 E16.5 ©

Specimen 3A Rb1-cKO^Blimp1 E16.5: embryonic day 16.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3A Rb1-cKO^Blimp1 E16.5

3A Rb1-cKO^Blimp1 E18.5 ©

Specimen 3A Rb1-cKO^Blimp1 E18.5: embryonic day 18.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		3A Rb1-cKO^Blimp1 E18.5

3D Control E16.5 ©

Specimen 3D E16.5 Control: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3D Control E16.5

3D Control P1.5 ©

Specimen 3D P1.5 Control: postnatal day 1.5 (more

)

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		3D Control P1.5

3D Control P3.5 ©

Specimen 3D P3.5 Control: postnatal day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		3D Control P3.5

3D Rb1-cKO^Blimp1 E16.5 ©

Specimen 3D E16.5 Rb1-cKO^Blimp1: embryonic day 16.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3D Rb1-cKO^Blimp1 E16.5

3D Rb1-cKO^Blimp1 P1.5 ©

Specimen 3D P1.5 Rb1-cKO^Blimp1: postnatal day 1.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		3D Rb1-cKO^Blimp1 P1.5	In this mutant the percentage of seminiferous cords devoid of germ cells increased through developmental time beginning with 11.2% at E18.5, and jumping to 87.2% at birth, compared to controls.

3D Rb1-cKO^Blimp1 P3.5 ©

Specimen 3D P6.5 Rb1-cKO^Blimp1: postnatal day 6.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present	Single cells	3D Rb1-cKO^Blimp1 P3.5	In this mutant the percentage of seminiferous cords devoid of germ cells was greater than 95% compared to controls.

3F Control E14.5 ©

Specimen 3F Control E14.5: embryonic day 14.5 (more

)
Note: Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3F Control E14.5

3F Control E16.5 ©

Specimen 3F Control E16.5: embryonic day 16.5 (more

)
Note: Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3F Control E16.5

Specimen 3F Control E18.5: embryonic day 18.5 (more

)
Note: Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		3F Control E18.5

Specimen 3F Rb1-cKO^Blimp1 E14.5: embryonic day 14.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS22: germ cell of testis	Present		3F Rb1-cKO^Blimp1 E14.5	Expression in this mutant is similar to controls.

Specimen 3F Rb1-cKO^Blimp1 E16.5: embryonic day 16.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		3F Rb1-cKO^Blimp1 E16.5

Specimen 3F Rb1-cKO^Blimp1 E18.5: embryonic day 18.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		3F Rb1-cKO^Blimp1 E18.5

Specimen 5G Control: embryonic day 16.5 (more

)
Note: Double labeled: red - Gcna; green - Stra8 (assay MGI:7662974).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		5G Control Merge , 5G Control TRA98

Specimen 5G Rb1-cKO^Blimp1: embryonic day 16.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Stra8 (assay MGI:7662974).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		5G Rb1-cKO^Blimp1 TRA98 , 5G Rb1-cKO^Blimp1 Merge

Specimen S2A Control: embryonic day 13.5 (more

)
Note: Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		S2A Control TRA98 , S2A Control Merge

Specimen S2A Rb1-cKO^Blimp1: embryonic day 13.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Mki67 (assay MGI:7662955).

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		S2A Rb1-cKO^Blimp1 TRA98 , S2A Rb1-cKO^Blimp1 Merge	Expression in this mutant is similar to controls.

Specimen S2C Control: embryonic day 15.5 (more

)
Note: Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS23: germ cell of testis	Present		S2C Control TRA98 , S2C Control Merge

Specimen S2C Rb1-cKO^Blimp1: embryonic day 15.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Casp3 (assay MGI:7662973).

Structure	Level	Pattern	Image	Note
TS23: germ cell of testis	Present		S2C Rb1-cKO^Blimp1 TRA98 , S2C Rb1-cKO^Blimp1 Merge	Expression in this mutant is similar to controls.

Specimen S7B Control: embryonic day 18.5 (more

)
Note: Double labeled: red - Gcna; green - Stra8 (assay MGI:7662974).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		S7B Control TRA98 , S7B Control Merge

Specimen S7B Rb1-cKO^Blimp1: embryonic day 18.5; Rb1^tm2Brn/Rb1^tm2Brn, Tg(Prdm1-cre)1Masu/0 (more

)
Note: Conditional mutant. Double labeled: red - Gcna; green - Stra8 (assay MGI:7662974).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		S7B Rb1-cKO^Blimp1 Merge , S7B Rb1-cKO^Blimp1 TRA98

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