Probe:	Dlx2 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: diencephalon floor plate	Ambiguous	Regionally restricted	6B E10.5 Dlx2	Expression wasn't described in the suprachiasmatic progenitor domain at E10.5.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Present	Regionally restricted	6B E11.5 Dlx2	Expression was first seen at E11.5 in a small band of ventral cells.

Structure	Level	Pattern	Image	Note
TS20: diencephalon floor plate	Present	Regionally restricted	6B E12.5 Dlx2	At E12.5, expression expanded dorsally both beneath as well as along the lateral aspects of the third ventricle.

Structure	Level	Pattern	Image	Note
TS21: diencephalon floor plate	Present	Regionally restricted	6B E13.5 Dlx2	At E13.5, expression expanded dorsally both beneath as well as along the lateral aspects of the third ventricle.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Present	Regionally restricted	6D E11.5 Rostral 1 Dlx2	Expression was seen in a small band of ventral cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Present	Regionally restricted	6D E11.5 2 Dlx2	Expression was seen in a small band of ventral cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Present	Regionally restricted	6D E11.5 3 Dlx2	Expression was seen in a small band of ventral cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Present	Regionally restricted	6D E11.5 4 Dlx2	Expression was seen in a small band of ventral cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon floor plate	Present	Regionally restricted	6D E11.5 Caudal 5 Dlx2	Expression was seen in a small band of ventral cells.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 Rostral 1 Dlx2	By E13.5 the Dlx2-expressing region expanded rostrocaudally and dorsally both beneath as well as along the lateral aspects of the third ventricle stratifying the region surrounding the base of the early third ventricle into three subregions.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 2 Dlx2	By E13.5 the Dlx2-expressing region expanded rostrocaudally and dorsally both beneath as well as along the lateral aspects of the third ventricle stratifying the region surrounding the base of the early third ventricle into three subregions.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 3 Dlx2	By E13.5 the Dlx2-expressing region expanded rostrocaudally and dorsally both beneath as well as along the lateral aspects of the third ventricle stratifying the region surrounding the base of the early third ventricle into three subregions.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 4 Dlx2	By E13.5 the Dlx2-expressing region expanded rostrocaudally and dorsally both beneath as well as along the lateral aspects of the third ventricle stratifying the region surrounding the base of the early third ventricle into three subregions.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 Caudal 5 Dlx2	By E13.5 the Dlx2-expressing region expanded rostrocaudally and dorsally both beneath as well as along the lateral aspects of the third ventricle stratifying the region surrounding the base of the early third ventricle into three subregions.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted		At E12.5 the most ventral expressing regions overlapped with Tubb3, a marker of postmitotic neurons, whereas the more dorsal Dlx2-negative region expressed Ki67, a marker of proliferating cells.