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Gene Expression Data
Assay Details
Assay
Reference: J:154534 Martinez G, et al., Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4794-806
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7660522
Gene symbol: Cdkn1c
Gene name: cyclin dependent kinase inhibitor 1C
Antibody: Anti-p57Kip2 (ab4058)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 8I: embryonic day 13.5 (more )
Note: Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS21: lens Present Regionally restricted 8I Expression was detected only in cells that had exited the cell cycle below the lens equator and decreased as fibers differentiated.
Specimen 8J: embryonic day 13.5; Ctnnb1tm1Mmt/Ctnnb1tm1Mmt, Tg(Cryaa-cre,Tyr)39Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS21: lens Present Regionally restricted 8J Expression was detected only in cells that had exited the cell cycle below the lens equator and decreased as fibers differentiated.
Specimen 8K: embryonic day 13.5; Ctnnb1tm1Mmt/Ctnnb1tm1Mmt, Tg(Cryaa-cre)10Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS21: lens Weak 8K Expression was delayed in cells below the equator. Many of the epithelioid cells below the equator were immunonegative.
Specimen 8L: embryonic day 13.5; Apctm1Tno/Apctm1Tno, Tg(Cryaa-cre)10Mlr/0 (more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Structure Level Pattern Image Note
TS21: lens Weak Regionally restricted 8L Expression was delayed in cells below the equator. Many of the epithelioid cells below the equator were immunonegative.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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