Gene Expression Data
Assay Details
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Assay
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Reference:
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J:154534
Martinez G, et al., Conditional mutations of beta-catenin and APC reveal roles for canonical Wnt signaling in lens differentiation. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4794-806
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7659205
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Antibody:
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Anti-Pax6
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
4E:
embryonic day 13.5
(more  )
Note: Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Specimen
4E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: lens |
lens epithelial cell |
Present |
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4E
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Expression was localized in the nuclei and cytoplasm of epithelial cells.
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| TS21: lens |
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Present |
Regionally restricted |
4E
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Expression was localized in the nuclei and cytoplasm of transitional zone cells.
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| TS21: lens fiber |
lens fiber cell |
Weak |
Regionally restricted |
4E
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Expression decreased rapidly in differentiating fiber cells below the equator.
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Specimen
4F:
embryonic day 13.5; Ctnnb1tm1Mmt/ Ctnnb1tm1Mmt, Tg(Cryaa-cre,Tyr)39Mlr/0
(more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Specimen
4F:
(close )
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Genetic Background:
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involves: 129X1/SvJ * FVB/N
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Mutant Allele(s):
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Ctnnb1tm1Mmt/Ctnnb1tm1Mmt
Tg(Cryaa-cre,Tyr)39Mlr/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: lens |
lens epithelial cell |
Present |
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4F
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Expression was localized in the nuclei and cytoplasm of epithelial cells.
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| TS21: lens |
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Present |
Regionally restricted |
4F
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Expression was localized in the nuclei and cytoplasm of transitional zone cells.
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| TS21: lens fiber |
lens fiber cell |
Weak |
Regionally restricted |
4F
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Expression decreased rapidly in differentiating fiber cells below the equator.
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Specimen
4G:
embryonic day 13.5; Ctnnb1tm1Mmt/ Ctnnb1tm1Mmt, Tg(Cryaa-cre)10Mlr/0
(more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Specimen
4G:
(close )
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Genetic Background:
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involves: 129X1/SvJ * FVB/N
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Mutant Allele(s):
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Ctnnb1tm1Mmt/Ctnnb1tm1Mmt
Tg(Cryaa-cre)10Mlr/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lens |
Present |
Widespread |
4G
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Nuclear expression was detected in all cells of the BetaCat<Ex3>10 lens.
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| TS21: lens fiber |
Present |
Regionally restricted |
4G
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Expression was detected in the epithelioid cells in the fiber cell compartment.
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Specimen
4H:
embryonic day 13.5; Apctm1Tno/ Apctm1Tno, Tg(Cryaa-cre)10Mlr/0
(more )
Note: Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
Specimen
4H:
(close )
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Genetic Background:
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involves: 129S4/SvJae * FVB/N
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Mutant Allele(s):
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Apctm1Tno/Apctm1Tno
Tg(Cryaa-cre)10Mlr/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Conditional mutant. Fixation was either 10% neutral buffered formalin or 4% paraformaldehyde. Embedding was either in paraffin or OCT (cryosection).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lens |
Present |
Widespread |
4H
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Nuclear expression was detected in all cells of the APC10 lens.
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| TS21: lens fiber |
Present |
Regionally restricted |
4H
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Expression was detected in the epithelioid cells in the fiber cell compartment.
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