Probe:	hMEF2C cDNA
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		1A/B
TS21: cortical subplate	Present		1A/B
TS21: telencephalon	Present	Regionally restricted	1A/B	Expression is in the superficial portion of the dorsal telencephalon.
TS21: cerebral cortex intermediate zone	Absent		1A/B
TS21: cerebral cortex ventricular layer	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong		1D/E
TS23: cortical subplate	Strong		1D/E
TS23: cerebral cortex intermediate zone	Absent		1D/E
TS23: cerebral cortex ventricular layer	Absent		1D/E

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Very strong	Non-Uniform	1G/H	Expression is in the superficial half, is in a deeper band with less expression, and another band with strong expression.
TS25: cortical subplate	Strong		1G/H
TS25: cerebral cortex intermediate zone	Absent		1G/H
TS25: cerebral cortex ventricular zone	Absent		1G/H

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Non-Uniform	2	Not all cells were strongly positive, others expressed weakly or not at all.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb	Present		3A/B
TS21: telencephalon	Present	Regionally restricted	3A/B	Expression is in the basal telencephalon.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb mitral cell layer	Strong		3C/D
TS23: olfactory cortex	Strong	Regionally restricted	3C/D	Expression is in the anterior olfactory nucleus.
TS23: main olfactory bulb layer	Present	Regionally restricted	3C/D	Expression is in the deeper differentiating field of the olfactory bulb which gives rise to the granular layer.
TS23: amygdala	Weak		3C/D
TS23: hypothalamus	Weak		3C/D
TS23: forebrain	Weak	Regionally restricted	3C/D	Expression is in some cells in more posterior portions of the basal forebrain.
TS23: telencephalon septum	Weak	Regionally restricted	3C/D	Expression is in septal nuclei.
TS23: main olfactory bulb ventricular layer	Absent		3C/D

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb mitral cell layer	Very strong		3E/F

Structure	Level	Pattern	Image	Note
TS25: amygdala	Present		5A/B
TS25: basal ganglia	Weak	Regionally restricted	5A/B	Expression is in the differentiating fields.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong	Regionally restricted	6A/B	Expression is in most cells of the thalamus.
TS25: hippocampus	Present	Regionally restricted	6A/B	Expression is in some cells.
TS25: reticular thalamic nucleus	Absent		6A/B

Structure	Level	Pattern	Image	Note
TS25: cerebellum Purkinje cell cluster	Strong		7A/B
TS25: inferior colliculus	Strong		7A/B
TS25: brainstem	Present	Regionally restricted	7A/B	Expression is in a small cluster.
TS25: cerebellum external granule cell layer	Absent		7A/B

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Weak	Regionally restricted		Expression is in a band in the anterior portion of the telencephalon. Absent in the olfactory bulb.
TS19: forebrain	Present	Regionally restricted		Expression is in the basal forebrain.

Structure	Level	Pattern	Image	Note
TS23: inferior colliculus	Present
TS23: thalamus	Present	Regionally restricted		Expression is in most cells of the thalamus.
TS23: reticular thalamic nucleus	Absent