About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:344836 Abou-Jaoude A, et al., Idax and Rinf facilitate expression of Tet enzymes to promote neural and suppress trophectodermal programs during differentiation of embryonic stem cells. Stem Cell Res. 2022 May;61:102770
Assay type: RT-PCR
MGI Accession ID: MGI:7641446
Gene symbol: Nes
Gene name: nestin
Probe: Nestin-pF, Nestin-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results Image: 4B Nestin
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
WT E12.5 TS20: forebrain Present Not Specified; total RNA involves: 129S4/SvJae * C57BL/6 Not Specified
Idax-/- E12.5 TS20: forebrain Present (a) Not Specified; total RNA involves: 129S4/SvJae * C57BL/6 Cxxc4em1Mmda/Cxxc4em1MmdaNot Specified
Rinf-/- E12.5 TS20: forebrain Present (a) Not Specified; total RNA involves: 129S4/SvJae * C57BL/6 Cxxc5em1Mmda/Cxxc5em1MmdaNot Specified
DKO E12.5 TS20: forebrain Present (b) Not Specified; total RNA involves: 129S4/SvJae * C57BL/6 Cxxc4em1Mmda/Cxxc4em1Mmda, Cxxc5em1Mmda/Cxxc5em1MmdaNot Specified
Notes:
(a) Expression levels are significantly reduced in the knock out compared to wild type.
(b) Expression levels are significantly reduced in the double knock out compared to wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory