Mpc1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:236179 Bowman CE, et al., Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. Mol Cell Biol. 2016 Aug 1;36(15):2089-104
Assay type:	RT-PCR
MGI Accession ID:	MGI:7628340
Gene symbol:	Mpc1
Gene name:	mitochondrial pyruvate carrier 1

Probe:	Mpc1-pF, Mpc1-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to 4 reference genes (18S rRNA, Rpl22, beta-actin, and Gapdh) and shown relative to wild type.

Results

Image: 6A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Cortex WT	E17.5	TS25: cerebral cortex	Present	0.01 µg; total RNA	involves: C57BL/6 * SJL		Not Specified
Cortex KI/+	E17.5	TS25: cerebral cortex	Weak (a)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1⁺	Not Specified
Cortex D/+	E17.5	TS25: cerebral cortex	Weak	0.01 µg; total RNA	involves: C57BL/6	Mpc1^tm1.2Mwol/Mpc1⁺	Not Specified
Cortex KI/KI	E17.5	TS25: cerebral cortex	Weak (b)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.1Mwol	Not Specified
Cortex D/KI	E17.5	TS25: cerebral cortex	Weak (c)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.2Mwol	Not Specified
Cortex KI/KI; Cre	E17.5	TS25: cerebral cortex	Weak (d)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.1Mwol, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Heart WT	E17.5	TS25: heart	Present	0.01 µg; total RNA	involves: C57BL/6 * SJL		Not Specified
Heart KI/+	E17.5	TS25: heart	Weak (a)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1⁺	Not Specified
Heart D/+	E17.5	TS25: heart	Weak	0.01 µg; total RNA	involves: C57BL/6	Mpc1^tm1.2Mwol/Mpc1⁺	Not Specified
Heart KI/KI	E17.5	TS25: heart	Weak (e)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.1Mwol	Not Specified
Heart D/KI	E17.5	TS25: heart	Weak (f)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.2Mwol	Not Specified
Heart KI/KI; Cre	E17.5	TS25: heart	Weak	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.1Mwol, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.
Liver WT	E17.5	TS25: liver	Present	0.01 µg; total RNA	involves: C57BL/6 * SJL		Not Specified
Liver KI/+	E17.5	TS25: liver	Weak (a)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1⁺	Not Specified
Liver D/+	E17.5	TS25: liver	Weak	0.01 µg; total RNA	involves: C57BL/6	Mpc1^tm1.2Mwol/Mpc1⁺	Not Specified
Liver KI/KI	E17.5	TS25: liver	Weak (g)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.1Mwol	Not Specified
Liver D/KI	E17.5	TS25: liver	Weak (h)	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.2Mwol	Not Specified
Liver KI/KI; Cre	E17.5	TS25: liver	Weak	0.01 µg; total RNA	involves: C57BL/6 * SJL	Mpc1^tm1.1Mwol/Mpc1^tm1.1Mwol, Tg(Nes-cre)1Kln/0	Not Specified	Conditional mutant.

Notes:
(a) Expression was dramatically reduced in the KI/+ animals.
(b) Expression was decreased in the brain (25% remaining).
(c) Expression was decreased in the brain (15% remaining).
(d) Expression was further reduced in the cortex (<4% of wild type).
(e) Expression was reduced in the heart to <12% of wild type levels remaining.
(f) Expression was reduced in the heart to 7% of wild type levels remaining.
(g) Expression was most severely decreased in the liver (11% remaining).
(h) Expression was reduced in the liver to 7% of wild type levels remaining.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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