Gene Expression Data
Assay Details
Assay
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Reference:
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J:345818
Petrova RS, et al., Verification of the gene and protein expression of the aquaglyceroporin AQP3 in the mammalian lens. Exp Eye Res. 2024 Mar;240:109828
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7628217
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Gene symbol:
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Aqp5
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Gene name:
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aquaporin 5
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Antibody:
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anti-AQP5
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
E10 not shown:
embryonic day 10.0
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
E10 not shown:
(close )
Genetic Background:
|
FVB/N
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: lens vesicle |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex.
|
Specimen
E11 not shown:
embryonic day 11.0
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
E11 not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: lens vesicle |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex.
|
Specimen
E16 not shown:
embryonic day 16.0
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
E16 not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
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Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lens core |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex and core.
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TS24: lens cortex |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex and core.
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Specimen
P3 not shown:
postnatal day 3
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
P3 not shown:
(close )
Genetic Background:
|
FVB/N
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: lens core |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex and core.
|
TS27: lens cortex |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex and core.
|
Specimen
P6 not shown:
postnatal day 6
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
P6 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens core |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex and core.
|
TS28: lens cortex |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex and core.
|
Specimen
P15 not shown:
postnatal day 15
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
P15 not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens cortex |
Present |
Regionally restricted |
|
Cytoplasmic expression in cortex.
|
TS28: lens core |
Present |
Regionally restricted |
|
Membranous expression in core.
|
Specimen
7G:
postnatal adult
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
7G:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: lens peripheral outer cortex |
lens fiber cell |
Present |
Regionally restricted |
7G
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Cytoplasmic expression restricted to peripheral fiber cells.
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Specimen
7H:
postnatal adult
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
7H:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: lens deep outer cortex |
lens fiber cell |
Present |
Regionally restricted |
7H
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Transition to membranous expression in differentiating fiber cells of deeper outer cortex.
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Specimen
7I:
postnatal adult
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
7I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: lens inner cortex |
secondary lens fiber |
Present |
Regionally restricted |
7I
|
Membranous expression in mature fiber cells of inner cortex.
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Specimen
7J:
postnatal adult
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
7J:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Specimen
7K:
postnatal adult
(more )
Note: Double labeled: green - Aqp5; red - WGA.
Specimen
7K:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Aqp5; red - WGA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens core |
Present |
Regionally restricted |
7K
|
Membranous expression in core.
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