Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80911
Mao GE, et al., Quantification and localization of expression of the retinoic acid receptor-beta and -gamma mRNA isoforms during neurulation in mouse embryos with or without spina bifida. Teratology. 2002 Dec;66(6):331-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7626303
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Gene symbol:
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Rarb
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Gene name:
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retinoic acid receptor, beta
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Probe:
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Rarb2 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A:
embryonic day 9.0
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B:
embryonic day 9.0
(more )
Specimen
4B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural tube |
Present |
Graded |
4B
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Expression extends caudally in closed neural tube, gradually diminishing to point where neural folds are open.
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| TS14: future spinal cord neural plate |
Absent |
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4B
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No expression open posterior neural folds.
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Specimen
4D:
embryonic day 9.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4D
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Expresssion in hindbrain caudal to otic vesicle.
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| TS15: neural tube |
Present |
Regionally restricted |
4D
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Expression in closed neural tube to sight of transition between open and closed neural tube.
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Specimen
4F:
embryonic day 10.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4H:
(close )
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Genetic Background:
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involves: C57BL * C57BL/6J
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
4H
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Expression in open posterior neuroepithelium, not restricted to closed neural tube in trunk region.
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Analysis Tools