H2ac1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:210169 Shinagawa T, et al., Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell. 2014 Feb 6;14(2):217-27
Assay type:	RT-PCR
MGI Accession ID:	MGI:7623728
Gene symbol:	H2ac1
Gene name:	H2A clustered histone 1

Probe:	Th2a-pF, Th2a-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

Image: 1A Th2a

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
MEF	Cultured cells: no data stored
Testis	P adult	TS28: testis	Present (a)	Not Specified; total RNA	involves: BALB/c		Male
Ovary	P adult	TS28: ovary	Absent	Not Specified; total RNA	involves: BALB/c		Female
Oocyte	P adult	TS28: oocyte	Present (b)	Not Specified; total RNA	involves: BALB/c		Female
one-cell	E0.5 (c)	TS1: 1-cell stage embryo	Present (d)	Not Specified; total RNA	involves: BALB/c		Not Specified
two-cell	E1.0 (c)	TS2: 2-cell stage embryo	Weak (e)	Not Specified; total RNA	involves: BALB/c		Not Specified
four-cell	E2.0 (c)	TS3: 4-cell stage embryo	Weak (e)	Not Specified; total RNA	involves: BALB/c		Not Specified
eight-cell	E2.5 (c)	TS3: 8-cell stage embryo	Trace (e)	Not Specified; total RNA	involves: BALB/c		Not Specified
ESC	Cultured cells: no data stored

Notes:
(a) High levels of expression detected,
(b) High levels of expression detected.
(c) Age assigned by curator based on morphological criteria supplied by authors.
(d) Significant levels of expression in zygote.
(e) Expression decreased as embryos differentiated.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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