Probe:	Slit2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: lens	Present		6c
TS21: optic nerve	Present		6c
TS21: optic stalk	Present		6c
TS21: retina ganglion cell layer	Present		6c
TS21: retina	Present	Regionally restricted	6c	Expression is in the outer edge of the retina (toward the periphery).

Structure	Level	Pattern	Image	Note
TS24: optic nerve	Present		6d
TS24: lens	Weak		6d
TS24: retina ganglion cell layer	Weak		6d
TS24: olfactory epithelium	Absent		6d

Structure	Level	Pattern	Image	Note
TS20: optic stalk	Present		7c , 7a
TS20: optic nerve	Present	Regionally restricted	7c , 7a	Expression is at the optic nerve head.

Structure	Level	Pattern	Image	Note
TS21: optic stalk	Present		7f , 7d
TS21: optic nerve	Present	Regionally restricted	7d , 7f	Expression is at the optic nerve head.

Structure	Level	Pattern	Image	Note
TS21: optic stalk	Present	Non-Uniform	7l , 7j , 7g , 7i	Expression is most prominent in cells throughout the ventricular lining within the center of the stalk.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Strong	Regionally restricted	8c , 8a	Expression is in regions anterior to the path that optic fibers take to the midline and is absent from posterior regions at which retinal fibers cross the ventral midline.
TS21: diencephalon	Present	Regionally restricted	8c , 8a	Expression is in the ventral diencephalon but is absent from the path of growing fibers.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Strong	Regionally restricted	8f , 8d	Expression is high in the ventral midline of the anterior portion of the hypothalamus and is absent further caudally where retinal ganglion cells axons cross the midline in the chiasm.
TS21: diencephalon	Present	Regionally restricted	8d , 8f	Expression is in the ventral diencephalon but is absent from the path of growing fibers.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Strong	Regionally restricted	8g , 8i	Expression is in regions anterior to the path that optic fibers take to the midline.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Strong	Regionally restricted	8j , 8l	Expression is high in the ventral midline of the anterior portion of the hypothalamus and is absent further caudally where retinal ganglion cells axons cross the midline in the chiasm.

Structure	Level	Pattern	Image	Note
TS21: optic stalk	Present	Regionally restricted		Expression is at the exit of the eye in dorsal stalk cells, between the pigment epithelial cells and the retinal ganglion cell axons.