Probe:	srGAP1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		1D
TS21: cerebral cortex intermediate zone	Weak		1D
TS21: cortical subplate	Weak		1D

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong		1G
TS24: cerebral cortex intermediate zone	Weak		1G
TS24: cerebral cortex ventricular layer	Weak		1G

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		1J
TS28: cortical layer III	Present		1J
TS28: cortical layer IV	Present		1J
TS28: cortical layer V	Present		1J
TS28: cortical layer VI	Present	Scattered	1J
TS28: cortical layer I	Absent		1J

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	2A	Expression is in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA3	Strong		2D
TS27: hippocampus	Present		2D
TS27: dentate gyrus	Weak		2D
TS27: hippocampus CA1	Weak		2D
TS27: dorsal thalamus	Weak		2D
TS27: thalamus	Weak		2D	Expression was weak in the dorsal and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		2G
TS28: dorsal lateral geniculate nucleus	Present		2G
TS28: hippocampus CA1	Present		2G
TS28: hippocampus CA3	Present		2G
TS28: ventral lateral geniculate nucleus	Weak		2G

Structure	Level	Pattern	Image	Note
TS21: midbrain roof plate	Present	Regionally restricted	3A	Expression is in the roof of the midbrain in the superior colliculus differentiating zone, and is largely absent from the superior colliculus neuroepithelium.
TS21: cerebellum	Absent		3A

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Absent		3A'

Structure	Level	Pattern	Image	Note
TS24: superior colliculus	Weak		3D
TS24: cerebellum	Weak	Regionally restricted	3D	Expression is in the cerebellar plate.
TS24: inferior colliculus	Weak		3D	There is a discrepancy between the table and the text. The text states that expression is present and the table states it is absent.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		3G
TS27: cerebellum external granule cell layer	Present		3G
TS27: cerebellum internal granule cell layer	Present		3G
TS27: tectum	Present	Regionally restricted	3G	Expression is in a region of the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		3J
TS28: cerebellum external granule cell layer	Strong		3J
TS28: cerebellum granule cell layer	Strong		3J
TS28: cerebellum molecular layer	Absent		3J

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	4A	Expression is in the ventral horns.
TS19: dorsal root ganglion	Absent		4A

Structure	Level	Pattern	Image	Note
TS20: spinal cord dorsal horn	Present		4D
TS20: spinal cord ventral horn	Present		4D

Structure	Level	Pattern	Image	Note
TS21: spinal cord dorsal horn	Present		4G
TS21: spinal cord ventral horn	Present		4G

Structure	Cell Type	Level	Pattern	Image	Note
TS22: main olfactory bulb mitral cell layer		Strong		5A
TS22: vomeronasal organ		Present		5A
TS22: main olfactory bulb layer	neuron	Present	Scattered	5A	Expression is in some scattered neurons of the ganglion layer.
TS22: olfactory epithelium		Absent		5A

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Strong		5D
TS27: main olfactory bulb granule cell layer	Present		5D
TS27: olfactory epithelium	Absent		5D

Structure	Level	Pattern	Image	Note
TS20: eye	Absent		6A

Structure	Level	Pattern	Image	Note
TS24: retina ganglion cell layer	Weak		6D

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted		Expression is in the ventral horn and is absent in the dorsal horn.
TS19: dorsal root ganglion	Absent
TS19: nasal pit	Absent
TS19: neural tube floor plate	Absent
TS19: neural tube roof plate	Absent
TS19: retina	Absent
TS19: telencephalon	Absent

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted		Expression is in the dorsal and ventral horn.
TS20: brain nucleus	Present	Regionally restricted		Expression is in the dorsal lateral geniculate nucleus and is absent in the ventral lateral geniculate nucleus.
TS20: cerebellum primordium	Present	Regionally restricted		Expression is in the cerebellar nuclei differentiating zone.
TS20: cerebral cortex ventricular layer	Absent
TS20: cortical preplate	Absent
TS20: dorsal root ganglion	Absent
TS20: olfactory epithelium	Absent
TS20: retina	Absent
TS20: spinal cord floor plate	Absent
TS20: spinal cord roof plate	Absent
TS20: vomeronasal organ	Absent

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present
TS21: cortical plate	Present
TS21: cortical subplate	Present
TS21: main olfactory bulb layer	Present	Regionally restricted		Expression is in the mitral layer and is absent in the granule cell layer.
TS21: brain nucleus	Present	Regionally restricted		Expression is in the dorsal lateral geniculate nucleus and ventral lateral geniculate nucleus.
TS21: midbrain roof plate	Present	Regionally restricted		Expression is in the differentiating zone of the superior colliculus.
TS21: spinal cord	Present	Regionally restricted		Expression is in the dorsal and ventral horn.
TS21: cerebral cortex marginal layer	Absent
TS21: cerebral cortex ventricular layer	Absent
TS21: dorsal root ganglion	Absent
TS21: olfactory epithelium	Absent
TS21: retina	Absent
TS21: spinal cord floor plate	Absent
TS21: spinal cord roof plate	Absent
TS21: vomeronasal organ	Absent

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present
TS22: cortical plate	Present
TS22: dorsal lateral geniculate nucleus	Present
TS22: hippocampus	Present
TS22: main olfactory bulb mitral cell layer	Present
TS22: retina ganglion cell layer	Present
TS22: ventral lateral geniculate nucleus	Present
TS22: vomeronasal organ	Present
TS22: neural retinal epithelium	Present	Regionally restricted		Expression is in the neuroblastic layer.
TS22: main olfactory bulb layer	Present	Regionally restricted		Expression is in the granule cell layer.
TS22: superior colliculus	Present	Regionally restricted		Expression is in the differentiating zone of the superior colliculus.
TS22: cerebral cortex marginal layer	Absent
TS22: cerebral cortex ventricular layer	Absent
TS22: cortical subplate	Absent
TS22: inferior colliculus	Absent
TS22: olfactory epithelium	Absent

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present
TS24: cerebral cortex ventricular layer	Present
TS24: cortical plate	Present
TS24: dorsal lateral geniculate nucleus	Present
TS24: hippocampus	Present
TS24: main olfactory bulb mitral cell layer	Present
TS24: main olfactory bulb prospective granule cell layer	Present
TS24: retina ganglion cell layer	Present
TS24: retina nuclear layer	Present
TS24: ventral lateral geniculate nucleus	Present
TS24: superior colliculus	Present	Regionally restricted		Expression is in the differentiating zone of the superior colliculus.
TS24: cerebellum	Present	Regionally restricted		Expression is in the cerebellar plate.
TS24: cerebral cortex marginal layer	Absent
TS24: cortical subplate	Absent
TS24: olfactory epithelium	Absent
TS24: vomeronasal organ	Absent
TS24: inferior colliculus	Absent			There is a discrepancy between the table and the text. The text states that expression is present and the table states it is absent.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present
TS27: cerebellum external granule cell layer	Present
TS27: cerebellum granule cell layer	Present
TS27: cortical layer II	Present
TS27: cortical layer III	Present
TS27: cortical layer IV	Present
TS27: cortical layer V	Present
TS27: cortical layer VI	Present
TS27: dentate gyrus	Present
TS27: dorsal lateral geniculate nucleus	Present
TS27: hippocampus CA1	Present
TS27: hippocampus CA3	Present
TS27: main olfactory bulb granule cell layer	Present
TS27: main olfactory bulb mitral cell layer	Present
TS27: ventral lateral geniculate nucleus	Present
TS27: inferior colliculus	Present	Regionally restricted		Expression is in the differentiating zone.
TS27: cerebellum molecular layer	Absent
TS27: cerebral cortex ventricular zone	Absent
TS27: cortical layer I	Absent
TS27: olfactory epithelium	Absent
TS27: superior colliculus	Absent
TS27: vomeronasal organ	Absent

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebellum external granule cell layer	Present
TS28: cerebellum granule cell layer	Present
TS28: cortical layer II	Present
TS28: cortical layer III	Present
TS28: cortical layer IV	Present
TS28: cortical layer V	Present
TS28: cortical layer VI	Present
TS28: dentate gyrus	Present
TS28: dorsal lateral geniculate nucleus	Present
TS28: hippocampus CA1	Present
TS28: hippocampus CA3	Present
TS28: ventral lateral geniculate nucleus	Present
TS28: ventral posterior lateral thalamic nucleus	Present
TS28: ventrolateral thalamic nucleus	Present
TS28: cerebellum molecular layer	Absent
TS28: cerebral cortex ventricular zone	Absent
TS28: cortical layer I	Absent
TS28: deep cerebellar nucleus	Absent