Gli1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:213418 Li D, et al., Formation of proximal and anterior limb skeleton requires early function of Irx3 and Irx5 and is negatively regulated by Shh signaling. Dev Cell. 2014 Apr 28;29(2):233-40
Assay type:	RT-PCR
MGI Accession ID:	MGI:7616240
Gene symbol:	Gli1
Gene name:	GLI-Kruppel family member GLI1

Probe:	Gli1-pF, Gli1-pR
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

Image: 4J

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Forelimb	E10.0 (a)	TS16: forelimb bud	Present	Not Specified	Not Specified		Not Specified	31-32-somite stage
Hindlimb	E10.5 (a)	TS17: hindlimb bud	Present (b)	Not Specified	Not Specified		Not Specified	36-37-somite stage

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Higher expression compared with forelimb buds at comparable stages (the hindlimb is delayed about 5-somite stage compared with the forelimb).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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